Genome Sequence of Trypanosoma cruzi, Etiologic Agent of Chagas Disease

• Published in: Science (American Association for the Advancement of Science) Vol. 309; no. 5733; pp. 409 - 415
• Main Authors: El-Sayed, Najib M, Myler, Peter J, Bartholomeu, Daniella C, Nilsson, Daniel, Aggarwal, Gautam, Tran, Anh-Nhi, Ghedin, Elodie, Worthey, Elizabeth A, Delcher, Arthur L, Blandin, Gae̊lle, Westenberger, Scott J, Caler, Elisabet, Cerqueira, Gustavo C, Branche, Carole, Haas, Brian, Anupama, Atashi, Arner, Erik, Åslund, Lena, Attipoe, Philip, Bontempi, Esteban, Bringaud, Frédéric, Burton, Peter, Cadag, Eithon, Campbell, David A, Carrington, Mark, Crabtree, Jonathan, Darban, Hamid, da Silveira, Jose Franco, de Jong, Pieter, Edwards, Kimberly, Englund, Paul T, Fazelina, Gholam, Feldblyum, Tamara, Ferella, Marcela, Frasch, Alberto Carlos, Gull, Keith, Horn, David, Hou, Lihua, Huang, Yiting, Kindlund, Ellen, Klingbeil, Michele, Kluge, Sindy, Koo, Hean, Lacerda, Daniela, Levin, Mariano J, Lorenzi, Hernan, Louie, Tin, Machado, Carlos Renato, McCulloch, Richard, McKenna, Alan, Mizuno, Yumi, Mottram, Jeremy C, Nelson, Siri, Ochaya, Stephen, Osoegawa, Kazutoyo, Pai, Grace, Parsons, Marilyn, Pentony, Martin, Pettersson, Ulf, Pop, Mihai, Ramirez, Jose Luis, Rinta, Joel, Robertson, Laura, Salzberg, Steven L, Sanchez, Daniel O, Seyler, Amber, Sharma, Reuben, Shetty, Jyoti, Simpson, Anjana J, Sisk, Ellen, Tammi, Martti T, Tarleton, Rick, Teixeira, Santuza, Van Aken, Susan, Vogt, Christy, Ward, Pauline N, Wickstead, Bill, Wortman, Jennifer, White, Owen, Fraser, Claire M, Stuart, Kenneth D, Andersson, Björn
• Format: Journal Article
• Language: English
• Published: United States American Association for the Advancement of Science 15.07.2005; The American Association for the Advancement of Science
• Subjects: Algorithms; Animals; Biologi; Biology; Cell and molecular biology; Cell- och molekylärbiologi; Chagas disease; Chagas Disease - drug therapy; Chagas Disease - parasitology; Chagas Disease/drug therapy/parasitology; Coding; diploidy; Disease; DNA; DNA Repair; DNA Replication; DNA, Mitochondrial - genetics; DNA, Protozoan - genetics; Eukaryotes; Eukaryotic cells; Genes; Genes, Protozoan; Genetic aspects; Genetics; Genetik; Genome, Protozoan; Genomes; Genomics; Haplotypes; Humans; Leishmania major; Meiosis; Membrane proteins; Membrane Proteins - chemistry; Membrane Proteins - genetics; Membrane Proteins - physiology; Membrane Proteins/chemistry/genetics/physiology; Mitochondrial DNA; Mucins; Multigene Family; NATURAL SCIENCES; NATURVETENSKAP; Parasites; Parasitic protozoa; Parasitism; pathogens; protein kinases; Proteins; Protozoan Proteins - chemistry; Protozoan Proteins - genetics; Protozoan Proteins - physiology; Protozoan Proteins/chemistry/genetics/physiology; Recombination, Genetic; Repetitive Sequences, Nucleic Acid; Retroelements; Retrotransposons; sequence analysis; Sequence Analysis, DNA; Signal Transduction; surface proteins; Telomere - genetics; Tribes; Trypanocidal Agents - pharmacology; Trypanocidal Agents - therapeutic use; Trypanocidal Agents/pharmacology/therapeutic use; Trypanosoma cruzi; Trypanosoma cruzi - chemistry; Trypanosoma cruzi - genetics; Trypanosoma cruzi - physiology; Trypanosoma cruzi/chemistry/genetics/physiology; Vector-borne diseases; Recombination; Research Support; N.I.H; Repetitive Sequences; Genes; Protozoan/genetics; Genetic; Protozoan; U.S. Gov't; Mitochondrial/genetics; Nucleic Acid; Extramural; P.H.S; DNA; Sequence Analysis; Genome; Non-U.S. Gov't
• ISSN: 0036-8075; 1095-9203; 1095-9203
• DOI: 10.1126/science.1112631

Whole-genome sequencing of the protozoan pathogen Trypanosoma cruzi revealed that the diploid genome contains a predicted 22,570 proteins encoded by genes, of which 12,570 represent allelic pairs. Over 50% of the genome consists of repeated sequences, such as retrotransposons and genes for large families of surface molecules, which include trans-sialidases, mucins, gp63s, and a large novel family (>1300 copies) of mucin-associated surface protein (MASP) genes. Analyses of the T. cruzi, T. brucei, and Leishmania major (Tritryp) genomes imply differences from other eukaryotes in DNA repair and initiation of replication and reflect their unusual mitochondrial DNA. Although the Tritryp lack several classes of signaling molecules, their kinomes contain a large and diverse set of protein kinases and phosphatases; their size and diversity imply previously unknown interactions and regulatory processes, which may be targets for intervention.