The distinct effects of orally administered Lactobacillus rhamnosus GG and Lactococcus lactis subsp. lactis C59 on gene expression in the murine small intestine

• Published in: PLOS ONE Vol. 12; no. 12; p. e0188985
• Main Authors: Suzuki, Chise, Aoki-Yoshida, Ayako, Aoki, Reiji, Sasaki, Keisuke, Takayama, Yoshiharu, Mizumachi, Koko
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science (PLoS) 08.12.2017; Public Library of Science
• Subjects: Administration, Oral; Agriculture; Analysis; Animals; Bacteria; Bioinformatics; Biological activity; Biological effects; Biology and Life Sciences; Data processing; Deoxyribonucleic acid; Disease; DNA; DNA microarrays; Duodenum; Ecosystems; Fibrinogen; Food; Gene Expression; Genes; Grasslands; Ileum; Intestine, Small; Lactic acid; Lactic acid bacteria; Lacticaseibacillus rhamnosus; Lactobacillus; Lactobacillus rhamnosus; Lactococcus lactis; Livestock; Lymphocytes B; Male; Medicine; Medicine and Health Sciences; Mice; Mice, Inbred BALB C; Microbiota; Microorganisms; Molecular modelling; Oral administration; Physical Sciences; Probiotics; Q; R; Research and Analysis Methods; Research Article; Ribonucleic acid; RNA; Science; Small intestine; Strains (organisms); Studies; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0188985

The molecular mechanisms of strain-specific probiotic effects and the impact of the oral administration of probiotic strains on the host's gene expression are not yet well understood. The aim of this study was to investigate the strain-specific effects of probiotic strain intake on gene expression in the murine small intestine. Two distinct strains of lactic acid bacteria, Lactobacillus rhamnosus GG (GG) and Lactococcus lactis subsp. lactis C59 (C59), were orally administered to BALB/c mice, daily for 2 weeks. The total RNA was isolated from the upper (including the duodenum) and lower (the terminal ileum) small intestine, and gene expression was assessed by microarray analysis. The data revealed (1) oral administration of C59 and GG markedly down-regulated the expression of genes encoding fibrinogen subunits and plasminogen in the upper small intestine; (2) administration of more than 1 × 107 CFU/day of GG changed the gene expression of the host ileum. (3) strain- and dose-related effects on various GO biological processes; and (4) enrichment for B cell-related Gene Ontology terms among up-regulated genes in the terminal ileum of mice administered the 1 × 109 CFU/day of GG. The distinct effects of GG and C59 on gene expression in the intact small intestine provide clues to understand how the health beneficial effects of specific strains of probiotic bacteria are mediated by interactions with intestinal cells.