γ显像在层析分离及蛋白分子位点密度测定中的应用

本文用放射性核素标记以及单光子发射型计算机断层（SPECT）γ显像的方法,进行E-选择素蛋白分子位点密度测定,以探讨其实用价值。采用Indogen法对E-选择素蛋白分子抗体进行125I标记,标记产物经G25葡聚糖凝胶层析柱分离纯化,对分离出的样品排列在塑胶试管架上,用SPECT进行平面显像并定量分析。制备不同浓度的E-选择素标准溶液,将放射性计数换算为位点密度,作蛋白量位点密度标准曲线。标记产物经SPECT定量分析,E-选择素抗体标记率为78%;不同浓度样品饱和曲线显示,当浓度在0-1 mg/m L浓度范围时,得标准曲线为y=6 045.7x—51.166,决定系数R^2=0.997 9。研究...

Full description

Saved in:
Bibliographic Details
Published inSheng wu yi xue gong cheng xue za zhi Vol. 34; no. 3; pp. 445 - 448
Main Author 张金赫 李趣欢 凌颖琛 钟建秋 尹吉林 徐浩
Format Journal Article
LanguageChinese
English
Published 中国四川 四川大学华西医院 25.06.2017
Subjects
E-选择素
位点密度
体层摄影术,发射型计算机,单光子
层析分离
新技术与新方法
Online AccessGet full text
ISSN1001-5515
DOI10.7507/1001-5515.201609035

Cover

More Information
Summary:本文用放射性核素标记以及单光子发射型计算机断层（SPECT）γ显像的方法,进行E-选择素蛋白分子位点密度测定,以探讨其实用价值。采用Indogen法对E-选择素蛋白分子抗体进行125I标记,标记产物经G25葡聚糖凝胶层析柱分离纯化,对分离出的样品排列在塑胶试管架上,用SPECT进行平面显像并定量分析。制备不同浓度的E-选择素标准溶液,将放射性计数换算为位点密度,作蛋白量位点密度标准曲线。标记产物经SPECT定量分析,E-选择素抗体标记率为78%;不同浓度样品饱和曲线显示,当浓度在0-1 mg/m L浓度范围时,得标准曲线为y=6 045.7x—51.166,决定系数R^2=0.997 9。研究结果表明,γ显像在放射性标记产物分析及位点密度测定时有一定实用价值。
Bibliography:tomography, emission-computed, single-photon; chromatography; E-selectin, site density
51-1258/R
ZHANG Jinhe1,2, LI Quhuan3, LING Yingchen3, ZHONG Jianqiu2, YIN Jilin2, XU Hao1 （1. Department of Nuclear Medicine, the First Affiliated Hospital,Jinan University, Guangzhou 510632, P.R.China; 2. Department of Nuclear Medicine, General Hospital of Guangzhou Military Command, Guangzhou 510010, P.R.China ;3. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510641, P.R. China）
We in this study measured the site density of E-selectin in order to explore the practical pliability using radionuclide labeling method and y-imaging of single photon emission computer tomography （SPECT）. This method required labeling of antibody with ^125I using Indogen method and binding of the labeled antibody to E-selectin. Labeled E- selectin was separated and purified in a Sephadex G25 column. The different fractions of the eluants were imaged, analyzed and quantified with SPECT method. For measuring
ISSN:1001-5515
DOI:10.7507/1001-5515.201609035