Antioxidant Activity of the Inclusion Complexes of Catechin, Epicatechin, Morin Hydrate, and Quercetin with Hydroxypropyl-β-Cyclodextrin

• Published in: Journal of the East Asian Society of Dietary Life Vol. 35; no. 2; pp. 135 - 145
• Main Author: Kim, Ji-Sang
• Format: Journal Article
• Language: English
• Published: 동아시아식생활학회 30.04.2025
• Subjects: 생활과학
• ISSN: 1225-6781; 2288-8802
• DOI: 10.17495/easdl.2025.4.35.2.135

This study aimed to formulate inclusion complexes of hydroxypropyl-β-cyclodextrin (HP-β-CD) with four flavonoids, namely, catechin (CA), epicatechin (EC), morin hydrate (MH), and quercetin (QC) and evaluated their antioxidant activities through in vitro assays. The antioxidant potential was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), oxygen radical absorbance capacity (ORAC), and hydrogen peroxide, hydroxyl, and superoxide radical scavenging assays. The antioxidant activity varied across the complexes and assays, largely influenced by the flavonoid structure, particularly the number and position of hydroxyl groups and their orientation within the cyclodextrin cavity. EC/HP-β-CD showed the highest DPPH activity (101.57 mM TE/g), while MH/HP-β-CD was most effective in the ABTS, FRAP, CUPRAC, and hydrogen peroxide assays, indicating strong reducing capacity. QC/HP-β-CD exhibited the highest hydroxyl and superoxide radical scavenging activity, highlighting its ability to neutralize reactive oxygen species. The HP-β-CD complexation improved the antioxidant performance of flavonoids by stabilizing reactive groups and enhancing solubility. These results emphasize the importance of the molecular structure in determining antioxidant efficiency and indicate that HP-β-CD inclusion complexes are promising candidates for antioxidant therapies and prevention of oxidative stress-related disorders. KCI Citation Count: 0