A pair of two－component regulatory genes ecrA1／A2 in S. coelicolor

• Published in: Journal of Zhejiang University. Science Vol. 5; no. 2; pp. 173 - 179
• Main Author: 李永泉 岑沛霖 陈时飞 吴凡 郑静
• Format: Journal Article
• Language: English
• Published: China 01.02.2004
• Subjects: Anthraquinones - metabolism; Chromosome Mapping - methods; DNA; Gene Expression Regulation, Bacterial - physiology; Genes, Regulator - genetics; Mutagenesis, Site-Directed; Prodigiosin - analogs & derivatives; Prodigiosin - biosynthesis; Streptomyces - genetics; Streptomyces - growth & development; Streptomyces - metabolism; Transcriptional Activation - genetics; 基因簇; 抗生素; 脱氧核糖核酸; 调节基因
• ISSN: 1009-3095
• DOI: 10.1007/BF02840919

Two-component genes are kinds of genetic elements involved in regulation of antibiotic production in Streptomyces coelicolor. DNA microarray analysis revealed that ecrA1/A2, which mapped at distant sites from red locus and encode respectively the kinase and regulator, expressed coordinately with genes of Red specific biosynthetic pathway, ecrA1 and ecrA2 gene-disruptive mutants were constructed using homogenotisation by reciprocal double crossover. Fermentation data showed that the undecylprodigiosin (Red) level of production was lower than that of wild-type strain. However, the change of the actinorhodin (Act) production level was not significant compared with wild type. Thus, these experiment results confirmed that the two-component system ecrA 1/A2 was positive regulatory element for red gene cluster.