高效液相色谱-串联质谱法测定蜂王浆中红霉素及其代谢物
目的建立蜂王浆中红霉素A、红霉素A烯醇醚、脱水红霉素A和N-脱甲基红霉素A的高效液相色谱-串联质谱检测方法。方法以pH 6.0磷酸盐缓冲液为提取剂,经HLB小柱净化富集后,采用高效液相色谱-串联质谱仪分析。采用Agilent Polaris C18色谱柱(100 mm×2.0 mm,5μm),以甲醇和0.1%甲酸为流动相,质谱模式为电喷雾正离子监测。结果该方法前处理简单,线性范围为2.0~100μg/L,相关系数在0.99以上,四种化合物的检出限和定量限分别为0.5μg/kg和5.0μg/kg。方法采用内标法定量,回收率范围为70.0~118.1%,相对标准偏差小于10%。结论方法回收率稳定且...
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| Published in | 食品安全质量检测学报 no. 10; pp. 2994 - 2999 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
江苏出入境检验检疫局动植物与食品检测中心国家蜂产品基准实验室,南京,210001
2014
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| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-0381 |
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| Abstract | 目的建立蜂王浆中红霉素A、红霉素A烯醇醚、脱水红霉素A和N-脱甲基红霉素A的高效液相色谱-串联质谱检测方法。方法以pH 6.0磷酸盐缓冲液为提取剂,经HLB小柱净化富集后,采用高效液相色谱-串联质谱仪分析。采用Agilent Polaris C18色谱柱(100 mm×2.0 mm,5μm),以甲醇和0.1%甲酸为流动相,质谱模式为电喷雾正离子监测。结果该方法前处理简单,线性范围为2.0~100μg/L,相关系数在0.99以上,四种化合物的检出限和定量限分别为0.5μg/kg和5.0μg/kg。方法采用内标法定量,回收率范围为70.0~118.1%,相对标准偏差小于10%。结论方法回收率稳定且重现性较好,能够满足日常检测工作的需要。 |
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| AbstractList | 目的：建立蜂王浆中红霉素A、红霉素A烯醇醚、脱水红霉素A和N-脱甲基红霉素A的高效液相色谱-串联质谱检测方法。方法以pH 6.0磷酸盐缓冲液为提取剂,经HLB小柱净化富集后,采用高效液相色谱-串联质谱仪分析。采用Agilent Polaris C18色谱柱(100 mm×2.0 mm,5μm),以甲醇和0.1%甲酸为流动相,质谱模式为电喷雾正离子监测。结果该方法前处理简单,线性范围为2.0~100μg/L,相关系数在0.99以上,四种化合物的检出限和定量限分别为0.5μg/kg和5.0μg/kg。方法采用内标法定量,回收率范围为70.0~118.1%,相对标准偏差小于10%。结论方法回收率稳定且重现性较好,能够满足日常检测工作的需要。 目的建立蜂王浆中红霉素A、红霉素A烯醇醚、脱水红霉素A和N-脱甲基红霉素A的高效液相色谱-串联质谱检测方法。方法以pH 6.0磷酸盐缓冲液为提取剂,经HLB小柱净化富集后,采用高效液相色谱-串联质谱仪分析。采用Agilent Polaris C18色谱柱(100 mm×2.0 mm,5μm),以甲醇和0.1%甲酸为流动相,质谱模式为电喷雾正离子监测。结果该方法前处理简单,线性范围为2.0~100μg/L,相关系数在0.99以上,四种化合物的检出限和定量限分别为0.5μg/kg和5.0μg/kg。方法采用内标法定量,回收率范围为70.0~118.1%,相对标准偏差小于10%。结论方法回收率稳定且重现性较好,能够满足日常检测工作的需要。 |
| Abstract_FL | Objective To established a method for determination of erythromycin A, erythromycin A enol ether, anhydroerythromycin A and N-demethyl erythromycin A by high performance liquid chromatogra-phy-tandem mass spectrometry.Methods The sample was extracted with pH 6.0 phosphate buffer and cleaned-up by HLB column and then analyzed by LC-MS with electrospray positive ionization. Separations were performed on an Agilent Polaris C18 column (100 mm×2.0 mm, 5μm). The mobile phase was methanol- 0.1% formic acid.Results Under the optimal conditions, the calibration curves were linear in the ranges of 2.0~100.0μg/L with the correlation coefficients more than 0.99 while the limit of detection and limit of quantitation were 0.5 and 5.0μg/kg, respectively. The internal standard was used for quantitative analysis. The recoveries ranged from 70.0 % to 118.1 % with RSDs less than 10%.Conclusion The method with higher recoveries and better precision could meet the needs of practical analysis. |
| Author | 张晓燕 朱文君 刘艳 陈磊 殷耀 黄娟 陈惠兰 吴斌 沈崇钰 |
| AuthorAffiliation | 江苏出入境检验检疫局动植物与食品检测中心 国家蜂产品基准实验室 |
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| Author_FL | YIN Yao ZHU Wen-Jun CHEN Lei CHEN Hui-Lan LIU Yan WU Bin ZHANG Xiao-Yan HUANG Juan SHEN Chong-Yu |
| Author_FL_xml | – sequence: 1 fullname: ZHANG Xiao-Yan – sequence: 2 fullname: ZHU Wen-Jun – sequence: 3 fullname: LIU Yan – sequence: 4 fullname: CHEN Lei – sequence: 5 fullname: YIN Yao – sequence: 6 fullname: HUANG Juan – sequence: 7 fullname: CHEN Hui-Lan – sequence: 8 fullname: WU Bin – sequence: 9 fullname: SHEN Chong-Yu |
| Author_xml | – sequence: 1 fullname: 张晓燕 朱文君 刘艳 陈磊 殷耀 黄娟 陈惠兰 吴斌 沈崇钰 |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitle_FL | Determination of erythromycin A and its metabolites in royal jelly by high performance liquid chromatography-tandem mass spectrometry |
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| Keywords | 代谢物 metabolite 蜂王浆 erythromycin high performance liquid chromatography-tandem mass spectrometry 红霉素 液相色谱-串联质谱法 royal jelly |
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| Notes | ZHANG Xiao-Yan;ZHU Wen-Jun;LIU Yan;CHEN Lei;YIN Yao;HUANG Juan;CHEN Hui-Lan;WU Bin;SHEN Chong-Yu;National Honey Reference Laboratory,Animal, Plant and Food Inspection Center (APFIC) of Jiangsu Entry-Exit Inspection and Quarantine Bureau 11-5956/TS |
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| PublicationTitle | 食品安全质量检测学报 |
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| PublicationYear | 2014 |
| Publisher | 江苏出入境检验检疫局动植物与食品检测中心国家蜂产品基准实验室,南京,210001 |
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| Snippet | 目的建立蜂王浆中红霉素A、红霉素A烯醇醚、脱水红霉素A和N-脱甲基红霉素A的高效液相色谱-串联质谱检测方法。方法以pH 6.0磷酸盐缓冲液为提取剂,经HLB小柱净化富集后,采用高效... 目的：建立蜂王浆中红霉素A、红霉素A烯醇醚、脱水红霉素A和N-脱甲基红霉素A的高效液相色谱-串联质谱检测方法。方法以pH 6.0磷酸盐缓冲液为提取剂,经HLB小柱净化富集后,采用高... |
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| Title | 高效液相色谱-串联质谱法测定蜂王浆中红霉素及其代谢物 |
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