Exome-wide association study reveals novel susceptibility genes to sporadic dilated cardiomyopathy

• Published in: PloS one Vol. 12; no. 3; p. e0172995
• Main Authors: Esslinger, Ulrike, Garnier, Sophie, Korniat, Agathe, Proust, Carole, Kararigas, Georgios, Müller-Nurasyid, Martina, Empana, Jean-Philippe, Morley, Michael P., Perret, Claire, Stark, Klaus, Bick, Alexander G., Prasad, Sanjay K., Kriebel, Jennifer, Li, Jin, Tiret, Laurence, Strauch, Konstantin, O'Regan, Declan P., Marguiles, Kenneth B., Seidman, Jonathan G., Boutouyrie, Pierre, Lacolley, Patrick, Jouven, Xavier, Hengstenberg, Christian, Komajda, Michel, Hakonarson, Hakon, Isnard, Richard, Arbustini, Eloisa, Grallert, Harald, Cook, Stuart A., Seidman, Christine E., Regitz-Zagrosek, Vera, Cappola, Thomas P., Charron, Philippe, Cambien, François, Villard, Eric
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 15.03.2017; Public Library of Science (PLoS)
• Subjects: Autophagy; Biology and Life Sciences; Cardiology; Cardiomyopathy; Cardiomyopathy, Dilated - genetics; Cardiovascular disease; Complications and side effects; Congestive cardiomyopathy; Diabetes; Dilated cardiomyopathy; Environmental health; Epidemiology; Exome; Gene expression; Gene mapping; Genes; Genetic aspects; Genetic Predisposition to Disease; Genetics; Genomics; Heart; Heart diseases; Heart failure; Hospitals; Humans; Kinases; Lead; Life Sciences; Loci; Medicine; Medicine and Health Sciences; Muscles; Mutation; Mutation, Missense; Nutrition; Polymorphism, Single Nucleotide; Research and Analysis Methods; Risk factors; Single nucleotide polymorphisms; Skeletal muscle; Tissues; France
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0172995

Dilated cardiomyopathy (DCM) is an important cause of heart failure with a strong familial component. We performed an exome-wide array-based association study (EWAS) to assess the contribution of missense variants to sporadic DCM. 116,855 single nucleotide variants (SNVs) were analyzed in 2796 DCM patients and 6877 control subjects from 6 populations of European ancestry. We confirmed two previously identified associations with SNVs in BAG3 and ZBTB17 and discovered six novel DCM-associated loci (Q-value<0.01). The lead-SNVs at novel loci are common and located in TTN, SLC39A8, MLIP, FLNC, ALPK3 and FHOD3. In silico fine mapping identified HSPB7 as the most likely candidate at the ZBTB17 locus. Rare variant analysis (MAF<0.01) demonstrated significant association for TTN variants only (P = 0.0085). All candidate genes but one (SLC39A8) exhibit preferential expression in striated muscle tissues and mutations in TTN, BAG3, FLNC and FHOD3 are known to cause familial cardiomyopathy. We also investigated a panel of 48 known cardiomyopathy genes. Collectively, rare (n = 228, P = 0.0033) or common (n = 36, P = 0.019) variants with elevated in silico severity scores were associated with DCM, indicating that the spectrum of genes contributing to sporadic DCM extends beyond those identified here. We identified eight loci independently associated with sporadic DCM. The functions of the best candidate genes at these loci suggest that proteostasis regulation might play a role in DCM pathophysiology.