Mechanism of Restoration of Forelimb Motor Function after Cervical Spinal Cord Hemisection in Rats: Electrophysiological Verification

• Published in: Behavioural Neurology Vol. 2017; no. 2017; pp. 1 - 11
• Main Authors: Ichimura, Shoichi, Hasegawa, Atsushi, Ohne, Hideaki, Satomi, Kazuhiko, Takahashi, Masahito, Takeuchi, Takumi, Sato, Shunsuke
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2017; Hindawi; Hindawi Limited; John Wiley & Sons, Inc; Wiley
• Subjects: Animals; Brain research; Experiments; Forelimb; Forelimb - physiology; Functional Laterality; Functional Laterality - physiology; Male; Motor Activity; Motor Activity - physiology; Motor Neurons; Neurons; Neurosciences. Biological psychiatry. Neuropsychiatry; Rats; Rats, Wistar; RC321-571; Recovery of Function; Recovery of Function - physiology; Research Article; Rodents; Spinal Cord; Spinal Cord Injuries; Spinal Cord Injuries - rehabilitation; Surgery; Upper Extremity; Upper Extremity - physiopathology; Japan
• ISSN: 0953-4180; 1875-8584; 1875-8584
• DOI: 10.1155/2017/7514681

The objective of this study was to electrophysiologically assess the corticospinal tracts of adult rats and the recovery of motor function of their forelimbs after cervical cord hemisection. Of 39 adult rats used, compound muscle action potentials (CMAPs) of the forelimbs of 15 rats were evaluated, before they received left C5 segmental hemisection of the spinal cord, by stimulating the pyramid of the medulla oblongata on one side using an exciting microelectrode. All 15 rats exhibited contralateral electrical activity, but their CMAPs disappeared after hemisection. The remaining 24 rats received hemisection first, and CMAPs of 12 rats were assessed over time to study their recovery time. All of them exhibited electrical activity of the forelimbs in 4 weeks after surgery. The remaining 12 rats received additional right C2 segmental hemisection, and variation of CMAPs between before and after surgery was examined. The right side of the 12 rats that received the additional hemisection exhibited no electrical activity in response to the stimulation of the pyramids on both sides. These results suggest that changes in path between the resected and healthy sides, activation of the ventral corticospinal tracts, and propriospinal neurons were involved in the recovery of motor function after cervical cord injury.