A Limited Number of Antibody Specificities Mediate Broad and Potent Serum Neutralization in Selected HIV-1 Infected Individuals

• Published in: PLoS pathogens Vol. 6; no. 8; p. e1001028
• Main Authors: Walker, Laura M., Simek, Melissa D., Priddy, Frances, Gach, Johannes S., Wagner, Denise, Zwick, Michael B., Phogat, Sanjay K., Poignard, Pascal, Burton, Dennis R.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.08.2010; Public Library of Science (PLoS)
• Subjects: Acquired immune deficiency syndrome; Adult; AIDS; AIDS Vaccines - immunology; Antibodies; Antibodies, Neutralizing - biosynthesis; Antibodies, Neutralizing - immunology; Antibody Specificity - immunology; Cross Reactions; env Gene Products, Human Immunodeficiency Virus - immunology; Epitopes, B-Lymphocyte - immunology; Female; Genetic diversity; HIV Antibodies - biosynthesis; HIV Antibodies - immunology; HIV Envelope Protein gp120 - immunology; HIV Envelope Protein gp41 - immunology; HIV infection; HIV-1 - immunology; Human immunodeficiency virus 1; Humans; Male; Neutralization (Chemistry); Physiological aspects; Vaccines; Viral antibodies; Virology/Immune Evasion; Virology/Immunodeficiency Viruses; Virology/Vaccines
• ISSN: 1553-7374; 1553-7366; 1553-7374
• DOI: 10.1371/journal.ppat.1001028

A protective vaccine against HIV-1 will likely require the elicitation of a broadly neutralizing antibody (bNAb) response. Although the development of an immunogen that elicits such antibodies remains elusive, a proportion of HIV-1 infected individuals evolve broadly neutralizing serum responses over time, demonstrating that the human immune system can recognize and generate NAbs to conserved epitopes on the virus. Understanding the specificities that mediate broad neutralization will provide insight into which epitopes should be targeted for immunogen design and aid in the isolation of broadly neutralizing monoclonal antibodies from these donors. Here, we have used a number of new and established technologies to map the bNAb specificities in the sera of 19 donors who exhibit among the most potent cross-clade serum neutralizing activities observed to date. The results suggest that broad and potent serum neutralization arises in most donors through a limited number of specificities (1-2 per donor). The major targets recognized are an epitope defined by the bNAbs PG9 and PG16 that is associated with conserved regions of the V1, V2 and V3 loops, an epitope overlapping the CD4 binding site and possibly the coreceptor binding site, an epitope sensitive to a loss of the glycan at N332 and distinct from that recognized by the bNAb 2G12 and an epitope sensitive to an I165A substitution. In approximately half of the donors, key N-linked glycans were critical for expression of the epitopes recognized by the bNAb specificities in the sera.