Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections

• Published in: PloS one Vol. 11; no. 10; p. e0164622
• Main Authors: Banar, Maryam, Emaneini, Mohammad, Satarzadeh, Mhboubeh, Abdellahi, Nafiseh, Beigverdi, Reza, Leeuwen, Willem B. van, Jabalameli, Fereshteh
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 13.10.2016; Public Library of Science (PLoS)
• Subjects: alpha-Mannosidase - adverse effects; alpha-Mannosidase - pharmacology; Analysis; Antibiotic resistance; Antibiotics; Antimicrobial agents; beta-Mannosidase - adverse effects; beta-Mannosidase - pharmacology; Biofilms; Biofilms - drug effects; Biology and Life Sciences; Burns; Burns (injuries); Burns - complications; Ceftazidime; Ceftazidime - pharmacology; Cell Line; Chemical compounds; Cross infection; Cytotoxicity; Drug resistance; Enzymes; Health aspects; Hospitals; Humans; Infections; Laboratories; Mannosidase; Mannosidases - adverse effects; Mannosidases - pharmacology; Medical research; Medicine; Medicine and Health Sciences; Methods; Microbial drug resistance; Microbial Sensitivity Tests; Nanoparticles; Nosocomial infections; Pharmacology; Pseudomonas aeruginosa; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - isolation & purification; Pseudomonas Infections - drug therapy; Pseudomonas Infections - microbiology; Research and Analysis Methods; Studies; Trypsin; Trypsin - pharmacology; Trypsin - therapeutic use; Tumor cell lines; Virulence; Virulence factors; Wound Infection - drug therapy; Wound Infection - microbiology; Wounds; Iran; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0164622

Biofilm is an important virulence factor in Pseudomonas aeruginosa and has a substantial role in antibiotic resistance and chronic burn wound infections. New therapeutic agents against P. aeruginosa, degrading biofilms in burn wounds and improving the efficacy of current antimicrobial agents, are required. In this study, the effects of α-mannosidase, β-mannosidase and trypsin enzymes on the degradation of P. aeruginosa biofilms and on the reduction of ceftazidime minimum biofilm eliminating concentrations (MBEC) were evaluated. All tested enzymes, destroyed the biofilms and reduced the ceftazidime MBECs. However, only trypsin had no cytotoxic effect on A-431 human epidermoid carcinoma cell lines. In conclusion, since trypsin had better features than mannosidase enzymes, it can be a promising agent in combatting P. aeruginosa burn wound infections.