Overexpression of Rice Wall-Associated Kinase 25 (OsWAK25) Alters Resistance to Bacterial and Fungal Pathogens

• Published in: PloS one Vol. 11; no. 1; p. e0147310
• Main Authors: Harkenrider, Mitch, Sharma, Rita, De Vleesschauwer, David, Tsao, Li, Zhang, Xuting, Chern, Mawsheng, Canlas, Patrick, Zuo, Shimin, Ronald, Pamela C.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.01.2016; Public Library of Science (PLoS)
• Subjects: Arabidopsis; Arabidopsis thaliana; Bacteria; BASIC BIOLOGICAL SCIENCES; Biology and Life Sciences; Epidermal growth factor; fungal pathogens; Fungi; Gene Expression Regulation, Plant; genetically modified plants; Genomes; Homology; hyperexpression techniques; Immunity, Innate - immunology; Infections; Kinases; Laboratories; leaves; Localization; Magnaporthe - pathogenicity; Magnaporthe grisea; Magnaporthe oryzae; Medicine and Health Sciences; Microbial drug resistance; Oryza; Oryza - immunology; Oryza - metabolism; Oryza - microbiology; Pathogenesis; Pathogenic microorganisms; Pathogens; Phenotype; Physiology; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - microbiology; Plant growth; Plant pathology; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified - immunology; Plants, Genetically Modified - metabolism; Plants, Genetically Modified - microbiology; protein domains; Proteins; Research and Analysis Methods; Rhizoctonia - pathogenicity; Rice; rice blast fungus; Risk factors; Signal transduction; Studies; Transcription; Wounding; Xanthomonas - pathogenicity; California; Belgium; United States > US; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0147310

Wall-associated kinases comprise a sub-family of receptor-like kinases that function in plant growth and stress responses. Previous studies have shown that the rice wall-associated kinase, OsWAK25, interacts with a diverse set of proteins associated with both biotic and abiotic stress responses. Here, we show that wounding and BTH treatments induce OsWAK25 transcript expression in rice. We generated OsWAK25 overexpression lines and show that these lines exhibit a lesion mimic phenotype and enhanced expression of rice NH1 (NPR1 homolog 1), OsPAL2, PBZ1 and PR10. Furthermore, these lines show resistance to the hemibiotrophic pathogens, Xanthomonas oryzae pv. oryzae (Xoo) and Magnaporthe oryzae, yet display increased susceptibility to necrotrophic fungal pathogens, Rhizoctonia solani and Cochliobolus miyabeanus.