Cytotoxic Constituents from Podocarpus fasciculus

• Published in: Chemical & Pharmaceutical Bulletin Vol. 56; no. 4; pp. 585 - 588
• Main Authors: Liao, Chia-Ching, Liang, Yu-Han, Hwang, Shy-Yuan, Wu, Ming-Der, Kuo, Yao-Haur, Ho, Hsiu-O, Kuo, Yu-Jen
• Format: Journal Article
• Language: English
• Published: TOKYO The Pharmaceutical Society of Japan 01.04.2008; Pharmaceutical Society of Japan; Pharmaceutical Soc Japan; Japan Science and Technology Agency
• Subjects: 16-hydroxy communic acid; Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - pharmacology; Carbohydrate Conformation; Cell Line, Tumor; Chemistry; Chemistry, Medicinal; Chemistry, Multidisciplinary; Chromatography, High Pressure Liquid; Coniferophyta - chemistry; cytotoxic activity; Diterpenes - chemistry; Diterpenes - pharmacology; Drug Screening Assays, Antitumor; Humans; Indicators and Reagents; Life Sciences & Biomedicine; Pharmacology & Pharmacy; Physical Sciences; Podocarpaceae; Podocarpus; Podocarpus fasciculus; Science & Technology; Spectrometry, Mass, Electrospray Ionization; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet; Structure-Activity Relationship; Tetrazolium Salts; Thiazoles; podocarpus fasciculus; MAGNETIC-RESONANCE-SPECTROSCOPY; DITERPENOIDS; DILACTONES; LEAVES; 16-hydroxy communic acid; SUBSTANCES; Podocarpaceae; cytotoxic activity; INHIBITORS
• ISSN: 0009-2363; 1347-5223; 1347-5223
• DOI: 10.1248/cpb.56.585

A new diterpene, 16-hydroxy communic acid (1), along with thirty one known compounds including five norditerpenes (2—6), twenty two flavonoids containing four biflavonoids (7—10), nine monoflavonoids (11—19) and nine flavanoid glycosides (20—28), as well as four phenolic constituents (29—32) were isolated from the 95% ethanolic extract of Podocarpus fasciculus. The structure of 1 was elucidated using spectral methods. Of these isolates, nagilactone C (2) showed the most significant inhibitory effects against DLD cells (human colon carcinoma) (ED50=2.57 μg/ml) and compounds 7, 8, 10, 11, and 12 had moderate cytotoxic activity against human KB (human oral epithelium carcinoma), Hela (human cervical carcinoma), Hepa (human hepatoma), DLD (colon carcinoma), and A-549 (human lung carcinoma) tumor cell lines. Preliminary structure–activity relationship studies of the isolated diterpenoids and biflavonoids are discussed.