Isolation and identification of milk oligosaccharide-degrading bacteria from the intestinal contents of suckling rats
We report the isolation of bacteria capable of degrading milk oligosaccharides from suckling infant rats. The bacteria were successfully isolated via a selective enrichment method, in which the serially diluted intestinal contents of infant rats were individually incubated in an enrichment medium co...
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Published in | Bioscience of Microbiota, Food and Health Vol. 40; no. 1; pp. 27 - 32 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Japan
BMFH Press
01.01.2021
Japan Science and Technology Agency |
Subjects | |
Online Access | Get full text |
ISSN | 2186-6953 2186-3342 2186-3342 |
DOI | 10.12938/bmfh.2020-024 |
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Summary: | We report the isolation of bacteria capable of degrading milk oligosaccharides from suckling infant rats. The bacteria were successfully isolated via a selective enrichment method, in which the serially diluted intestinal contents of infant rats were individually incubated in an enrichment medium containing 3′-sialyllactose (3′-SL), followed by the isolation of candidate strains from streaked agar plates and selection of 3′-SL-degrading strains using thin-layer chromatography. Subsequent genomic and phenotypic analyses identified all strains as Enterococcus gallinarum. The strains were capable of degrading both 3′-SL and 6′-SL, which was not observed with the type strain of E. gallinarum used as a reference. Furthermore, a time-course study combining high-performance anion-exchange chromatography with pulsed amperometric detection revealed that the representative strain AH4 degraded 3′-SL completely to yield an equimolar amount of lactose and an approximately one-fourth equimolar amount of sialic acid after 24 hr of anaerobic incubation. These findings point to a possibility that the enterococci degrade rat milk oligosaccharides to “cross-feed” their degradants to other members of concomitant bacteria in the gut of the infant rat. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
ISSN: | 2186-6953 2186-3342 2186-3342 |
DOI: | 10.12938/bmfh.2020-024 |