Evaluation of Tegaran Formula ZhenHua cytotoxicity against human cancer cell lines

• Published in: PloS one Vol. 15; no. 10; p. e0240969
• Main Authors: Parsonidis, Panagiotis, Vlachou, Ioanna, Mamagkaki, Alexandra, Bouris, Ioannis, Daikopoulou, Vasiliki, Papasotiriou, Ioannis
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.10.2020; Public Library of Science (PLoS)
• Subjects: Analytical methods; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Biology and Life Sciences; Cancer; Cancer treatment; Capsules; Cell Line, Tumor; Cell Survival - drug effects; Colorimetry; Cytotoxicity; Disintegration; Ethanol; Evaluation; Fermentation; Gas Chromatography-Mass Spectrometry; Glycine max - chemistry; HCT116 Cells; Health aspects; High-performance liquid chromatography; Humans; Liquid chromatography; MCF-7 Cells; Medicine and Health Sciences; Methods; Neoplasms - drug therapy; NMR; Nuclear magnetic resonance; Physical Sciences; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plants, Medicinal - chemistry; Research and analysis methods; Solubility; Solvents; Soy products; Soybean products; Toxicity; Tumor cell lines; Switzerland; Greece; United Kingdom > UK; United States > US; China; Germany
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0240969

The aim of this study is to evaluate the potential health effects of Tegaran Formula ZhenHua, a nutritional supplement used mainly by cancer patients. Its active ingredients and cytotoxicity was assessed with analytical methods and viability assays, respectively. The analytical methods consisted of dissolution, disintegration, HPLC, LC/MS, GC/MS and NMR. Cytotoxicity was assessed by MTT, SRB, CVE colorimetric viability assays in 0, 24, 48 and 72h time points. The results indicate that Tegaran Formula ZhenHua supplement did not present any cytotoxic effects due to issues related to the capsules' solubility, distribution and identification of the active ingredient.