The Impact of Full-Length, Trimeric and Globular Adiponectin on Lipolysis in Subcutaneous and Visceral Adipocytes of Obese and Non-Obese Women

• Published in: PloS one Vol. 8; no. 6; p. e66783
• Main Authors: Wedellova, Zuzana, Kovacova, Zuzana, Tencerova, Michaela, Vedral, Tomas, Rossmeislova, Lenka, Siklova-Vitkova, Michaela, Stich, Vladimir, Polak, Jan
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.06.2013; Public Library of Science (PLoS)
• Subjects: Adipocytes; Adipocytes - cytology; Adipocytes - drug effects; Adipocytes - metabolism; Adiponectin; Adiponectin - blood; Adiponectin - chemistry; Adiponectin - metabolism; Adipose tissue; Adult; Aminoimidazole Carboxamide - analogs & derivatives; Aminoimidazole Carboxamide - pharmacology; AMP; AMP-activated protein kinase; AMP-Activated Protein Kinases - metabolism; Biology; Cells, Cultured; Female; Gene Expression - drug effects; Glucose; Glycerol; Humans; Hypoglycemic Agents - pharmacology; Insulin resistance; Intra-Abdominal Fat - cytology; Isoforms; Kinases; Lipolysis; Lipolysis - drug effects; Medicine; Middle Aged; Obesity; Obesity - pathology; Preadipocytes; Protein Isoforms - blood; Protein Isoforms - chemistry; Protein Isoforms - metabolism; Protein Multimerization - drug effects; Real-Time Polymerase Chain Reaction; Receptors; Receptors, Adiponectin - genetics; Receptors, Adiponectin - metabolism; Ribonucleotides - pharmacology; Rodents; Subcutaneous Fat - cytology; Tissues; Type 2 diabetes; United States > US; Czech Republic
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0066783

Contribution of individual adiponectin isoforms to lipolysis regulation remains unknown. We investigated the impact of full-length, trimeric and globular adiponectin isoforms on spontaneous lipolysis in subcutaneous abdominal (SCAAT) and visceral adipose tissues (VAT) of obese and non-obese subjects. Furthermore, we explored the role of AMPK (5'-AMP-activated protein kinase) in adiponectin-dependent lipolysis regulation and expression of adiponectin receptors type 1 and 2 (AdipoR1 and AdipoR2) in SCAAT and VAT. Primary adipocytes isolated from SCAAT and VAT of obese and non-obese women were incubated with 20 µg/ml of: A) full-length adiponectin (physiological mixture of all adiponectin isoforms), B) trimeric adiponectin isoform or C) globular adiponectin isoform. Glycerol released into media was used as a marker of lipolysis. While full-length adiponectin inhibited lipolysis by 22% in non-obese SCAAT, globular isoform inhibited lipolysis by 27% in obese SCAAT. No effect of either isoform was detected in non-obese VAT, however trimeric isoform inhibited lipolysis by 21% in obese VAT (all p<0.05). Trimeric isoform induced Thr172 p-AMPK in differentiated preadipocytes from a non-obese donor, while globular isoform induced Ser79 p-ACC by 32% (p<0.05) and Ser565 p-HSL by 52% (p = 0.08) in differentiated preadipocytes from an obese donor. AdipoR2 expression was 17% and 37% higher than AdipoR1 in SCAAT of obese and non-obese groups and by 23% higher in VAT of obese subjects (all p<0.05). In conclusion, the anti-lipolytic effect of adiponectin isoforms is modified with obesity: while full-length adiponectin exerts anti-lipolytic action in non-obese SCAAT, globular and trimeric isoforms show anti-lipolytic activity in obese SCAAT and VAT, respectively.