Elevated plasma miRNA-122, -140-3p, -720, -2861, and -3149 during early period of acute coronary syndrome are derived from peripheral blood mononuclear cells

• Published in: PloS one Vol. 12; no. 9; p. e0184256
• Main Authors: Li, Xiang-Dong, Yang, Yue-Jin, Wang, Lai-Yuan, Qiao, Shu-Bin, Lu, Xiang-Feng, Wu, Yong-Jian, Xu, Bo, Li, Hong-Fan, Gu, Dong-Feng
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 29.09.2017; Public Library of Science (PLoS)
• Subjects: Acute coronary syndrome; Acute Coronary Syndrome - blood; Acute Coronary Syndrome - genetics; Acute coronary syndromes; Angina; Angina pectoris; Animals; Atherosclerosis; Biology and life sciences; Blood; Blood cells; Blood circulation; Blood platelets; Brain; Breast cancer; Cardiology; Cardiovascular disease; Coronary vessels; Endothelial cells; Epidemiology; Fibrillation; Gene expression; Gene Expression Profiling; Genetic aspects; Heart; Heart attacks; Hospitals; Humans; Ischemia; Kidneys; Laboratory animals; Leukocytes (mononuclear); Leukocytes, Mononuclear - metabolism; Liver; Lungs; Lymphocytes; Male; Medicine and Health Sciences; MicroRNA; MicroRNAs; MicroRNAs - blood; miRNA; Monocytes; Muscles; Myocardial infarction; Myocardium; Patients; Peripheral blood mononuclear cells; Plasmas (physics); Platelets; Real-Time Polymerase Chain Reaction; Ribonucleic acid; RNA; Skeletal muscle; Spleen; Swine; Swine, Miniature; Tissue Distribution; Ventricle; Ventricular fibrillation; Beijing China; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0184256

Our previous study has found that circulating microRNA (miRNA, or miR) -122, -140-3p, -720, -2861, and -3149 are significantly elevated during early stage of acute coronary syndrome (ACS). This study was conducted to determine the origin of these elevated plasma miRNAs in ACS. qRT-PCR was performed to detect the expression profiles of these 5 miRNAs in liver, spleen, lung, kidney, brain, skeletal muscles, and heart. To determine their origins, these miRNAs were detected in myocardium of acute myocardial infarction (AMI), and as well in platelets and peripheral blood mononuclear cells (PBMCs, including monocytes, circulating endothelial cells (CECs) and lymphocytes) of the AMI pigs and ACS patients. MiR-122 was specifically expressed in liver, and miR-140-3p, -720, -2861, and -3149 were highly expressed in heart. Compared with the sham pigs, miR-122 was highly expressed in the border zone of the ischemic myocardium in the AMI pigs without ventricular fibrillation (P < 0.01), miR-122 and -720 were decreased in platelets of the AMI pigs, and miR-122, -140-3p, -720, -2861, and -3149 were increased in PBMCs of the AMI pigs (all P < 0.05). Compared with the non-ACS patients, platelets miR-720 was decreased and PBMCs miR-122, -140-3p, -720, -2861, and -3149 were increased in the ACS patients (all P < 0.01). Furthermore, PBMCs miR-122, -720, and -3149 were increased in the AMI patients compared with the unstable angina (UA) patients (all P < 0.05). Further origin identification revealed that the expression levels of miR-122 in CECs and lymphocytes, miR-140-3p and -2861 in monocytes and CECs, miR-720 in monocytes, and miR-3149 in CECs were greatly up-regulated in the ACS patients compared with the non-ACS patients, and were higher as well in the AMI patients than that in the UA patients except for the miR-122 in CECs (all P < 0.05). The elevated plasma miR-122, -140-3p, -720, -2861, and -3149 in the ACS patients were mainly originated from CECs and monocytes.