Selective DNAM-1 expression on small peritoneal macrophages contributes to CD4+ T cell costimulation

• Published in: Scientific reports Vol. 8; no. 8; pp. 15180 - 8
• Main Authors: 渋谷 彰, 渋谷 和子, Takenaka Eri, Van Vo Anh, Yamashita-Kanemaru Yumi, Shibuya Akira, Shibuya Kazuko
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group 12.10.2018; Nature Publishing Group UK; Nature Portfolio
• Subjects: 13/31; 631/250/21; 631/250/2504/342/1956; 64/60; Alum Adjuvant; Aluminum sulfate; Antigen presentation; Antigens; CD11b antigen; CD226 antigen; CD4 antigen; Humanities and Social Sciences; Immunization; Lymph nodes; Lymphocytes; Lymphocytes B; Lymphocytes T; Macrophages; Mediastinal Lymph Nodes; MHC class-II (MHCII); multidisciplinary; Ovalbumin; Peritoneal Macrophages; Peritoneum; Science; Science (multidisciplinary); T cell receptors; Trinitrophenyl; Trinitrophenyl (TNP); Alum Adjuvant; Trinitrophenyl (TNP); Mediastinal Lymph Nodes; Peritoneal Macrophages; MHC class-II (MHCII)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-33437-4

Mouse peritoneal macrophages consist of two subsets: large peritoneal macrophages (LPMs) and small peritoneal macrophages (SPMs), defined as CD11bhiF4/80hi and CD11b+F4/80lo cells, respectively. We reveal that SPMs, but not LPMs, have the ability to present antigens to naïve CD4+ T cells. Coculture of SPMs with naïve ovalbumin (OVA) specific CD4+ T cells (OT-II) in the presence of OVA peptide effectively induced CD4+ T cells priming. SPMs, but not LPMs, strongly express DNAM-1, an activating immunoreceptor. Although antigen uptake and processing were comparable between WT and DNAM-1-deficient SPMs, deficiency of DNAM-1 on SPMs or blockade of DNAM-1 and its ligand interaction impaired CD4+ T cells priming by SPMs. Furthermore, T and B cell responses in mediastinal lymph nodes of mice intraperitoneally immunized with trinitrophenyl (TNP)–OVA protein in Alum adjuvant were enhanced by intraperitoneally transferred wild-type, but not DNAM-1-deficient, SPMs. We propose that SPMs are functionally distinct from LPMs, and DNAM-1 plays a costimulatory role in antigen presentation by SPMs.