A Proteomic Approach to Lipo-Chitooligosaccharide and Thuricin 17 Effects on Soybean GerminationUnstressed and Salt Stress

• Published in: PloS one Vol. 11; no. 8; p. e0160660
• Main Authors: Subramanian, Sowmyalakshmi, Ricci, Emily, Souleimanov, Alfred, Smith, Donald L.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 25.08.2016; Public Library of Science (PLoS)
• Subjects: Bacteriocins - pharmacology; Biology and Life Sciences; Dose-Response Relationship, Drug; Ecology and Environmental Sciences; Genetic aspects; Germination - drug effects; Glycine max - drug effects; Glycine max - growth & development; Glycine max - metabolism; Lipopolysaccharides - pharmacology; Mass Spectrometry; Physical Sciences; Physiological aspects; Plant Proteins - metabolism; Proteome - metabolism; Proteomics; Proteomics - methods; Sodium Chloride - pharmacology; Soil salinity; Soybeans; Stress, Physiological; Time Factors; Canada
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0160660

Salt stress is an important abiotic stressor affecting crop growth and productivity. Of the 20 percent of the terrestrial earth's surface available as agricultural land, 50 percent is estimated by the United Nations Environment Program to be salinized to the level that crops growing on it will be salt-stressed. Increased soil salinity has profound effects on seed germination and germinating seedlings as they are frequently confronted with much higher salinities than vigorously growing plants, because germination usually occurs in surface soils, the site of greatest soluble salt accumulation. The growth of soybean exposed to 40 mM NaCl is negatively affected, while an exposure to 80 mM NaCl is often lethal. When treated with the bacterial signal compounds lipo-chitooligosaccharide (LCO) and thuricin 17 (Th17), soybean seeds (variety Absolute RR) responded positively at salt stress of up to 150 mM NaCl. Shotgun proteomics of unstressed and 100 mM NaCl stressed seeds (48 h) in combination with the LCO and Th17 revealed many known, predicted, hypothetical and unknown proteins. In all, carbon, nitrogen and energy metabolic pathways were affected under both unstressed and salt stressed conditions when treated with signals. PEP carboxylase, Rubisco oxygenase large subunit, pyruvate kinase, and isocitrate lyase were some of the noteworthy proteins enhanced by the signals, along with antioxidant glutathione-S-transferase and other stress related proteins. These findings suggest that the germinating seeds alter their proteome based on bacterial signals and on stress, the specificity of this response plays a crucial role in organ maturation and transition from one stage to another in the plants' life cycle; understanding this response is of fundamental importance in agriculture and, as a result, global food security. The mass spectrometry proteomics data have been deposited to the ProteomeXchange with identifier PXD004106.