Reactive oxygen species-induced protein carbonylation promotes deterioration of physiological activity of wheat seeds

• Published in: PloS one Vol. 17; no. 3; p. e0263553
• Main Authors: Li, Bang-Bang, Zhang, Shuai-Bing, Lv, Yang-Yong, Wei, Shan, Hu, Yuan-Sen
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 31.03.2022; Public Library of Science (PLoS)
• Subjects: Aging; Aging (artificial); Agricultural production; Antioxidants; Antioxidants - metabolism; Bioengineering; Biology and Life Sciences; Carbonyl compounds; Carbonyls; Correlation; Cytology; Electron microscopy; Embryo cells; Embryos; Engineering; Engineering and Technology; Germination; Glutathione; Humidity; Oxidation; Oxygen; Physical Sciences; Properties; Protein Carbonylation; Proteins; Reactive oxygen species; Reactive Oxygen Species - metabolism; Regulatory mechanisms (biology); Seeds; Seeds - metabolism; Transmission electron microscopy; Triticum - metabolism; Vigor; Wheat; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0263553

During the seed aging process, reactive oxygen species (ROS) can induce the carbonylation of proteins, which changes their functional properties and affects seed vigor. However, the impact and regulatory mechanisms of protein carbonylation on wheat seed vigor are still unclear. In this study, we investigated the changes in wheat seed vigor, carbonyl protein content, ROS content and embryo cell structure during an artificial aging process, and we analyzed the correlation between protein carbonylation and seed vigor. During the artificial wheat-seed aging process, the activity levels of antioxidant enzymes and the contents of non-enzyme antioxidants decreased, leading to the accumulation of ROS and an increase in the carbonyl protein content, which ultimately led to a decrease in seed vigor, and there was a significant negative correlation between seed vigor and carbonyl protein content. Moreover, transmission electron microscopy showed that the contents of protein bodies in the embryo cells decreased remarkably. We postulate that during the wheat seed aging process, an imbalance in ROS production and elimination in embryo cells leads to the carbonylation of proteins, which plays a negative role in wheat seed vigor.