The excretory-secretory products of Echinococcus granulosus protoscoleces directly regulate the differentiation of B10, B17 and Th17 cells

• Published in: Parasites & vectors Vol. 10; no. 1; p. 348
• Main Authors: Pan, Wei, Hao, Wen-Ting, Shen, Yu-Juan, Li, Xiang-Yang, Wang, Yan-Juan, Sun, Fen-Fen, Yin, Jian-Hai, Zhang, Jing, Tang, Ren-Xian, Cao, Jian-Ping, Zheng, Kui-Yang
• Format: Journal Article
• Language: English
• Published: London BioMed Central 21.07.2017; BioMed Central Ltd; BMC
• Subjects: Animals; Antigens; Antigens, Helminth - immunology; Arthritis; B-Lymphocyte Subsets - immunology; Biomedical and Life Sciences; Biomedicine; Carbohydrates; CD19 antigen; CD4 antigen; CD4-Positive T-Lymphocytes - immunology; Cell Differentiation; Cells, Cultured; Cellular proteins; Cysts; Cytokines; Deactivation; Dendritic cells; Differentiation; Dog tapeworm; Echinococcosis - immunology; Echinococcosis - parasitology; Echinococcus granulosus; Echinococcus granulosus - immunology; Echinococcus granulosus - metabolism; Echinococcus granulosus protoscoleces; Entomology; Excretory-secretory products; Female; Gene expression; Genetic aspects; Health aspects; Heat; heat inactivation; Heat treatment; helminths; Helper cells; Host-Parasite Interactions; Host-parasite relationships; IL-10-producing B cells; IL-17A-producing B cells; Immune response regulation; In vitro methods and tests; Inactivation; Infections; Infectious Diseases; Inflammation; Interleukin 10; Interleukin-10 - biosynthesis; Interleukin-10 - genetics; interleukin-17; Interleukin-17 - biosynthesis; Interleukin-17 - genetics; Interleukins; Lymphocytes; Lymphocytes B; Lymphocytes T; Mice; Mice, Inbred BALB C; mRNA; Parasites; Parasitology; Penicillin; Profiles; protoscoleces; Secretory products; T cell receptors; T-lymphocytes; Th17 Cells - immunology; Th17 Cells - physiology; Tropical Medicine; Veterinary Medicine/Veterinary Science; Virology; United States > US; IL-17A-producing B cells; Excretory-secretory products; Inflammation; IL-10-producing B cells; protoscoleces; Echinococcus granulosus protoscoleces
• ISSN: 1756-3305; 1756-3305
• DOI: 10.1186/s13071-017-2263-9

Background Excretory-secretory products (ESPs) released by helminths are well-known to regulate T cell responses in the host. However, their direct influence in the differentiation of naïve T cells, and especially B cells, remains largely unknown. This study investigated the effects of Echinococcus granulosus protoscoleces ESPs (EgPSC-ESPs) on the differentiation of IL-10-producing B cells (B10), IL-17A-producing B cells (B17) and Th17 cells. Methods BALB/c mice injected with EgPSC were used to evaluate the in vivo profiles of B10, B17 and Th17 cells. In vitro purified CD19 + B and naïve CD4 + T cells were cultured in the presence of native, heat-inactivated or periodate-treated EgPSC-ESPs, and the differentiation of these cell subsets were compared. Results In contrast to the control group, infected mice showed higher frequencies of B10, B17 and Th17 cells, and higher levels of IL-10 and IL-17A in the sera. Interestingly, B17 cells were first identified to express CD19 + CD1d high . In vitro, B cells cultured with native ESPs exhibited a higher percentage of B10 cells but lower percentage of B17 and Th17 cells compared to the PBS group. Moreover, the relative expression of IL-10 and IL-17A mRNA were consistent with the altered frequencies. However, ESPs subjected to heat-inactivation or periodate treatment exhibited an inverse effect on the induction of these cell subsets. Conclusions Our findings indicate that ESPs released by EgPSC can directly regulate the differentiation of B10, B17 and Th17 cells, which appear to be heat-labile and carbohydrate-dependent.