pyDHM: A Python library for applications in digital holographic microscopy

• Published in: PloS one Vol. 17; no. 10; p. e0275818
• Main Authors: Castañeda, Raul, Trujillo, Carlos, Doblas, Ana
• Format: Journal Article
• Language: English
• Published: San Francisco Public Library of Science 10.10.2022; Public Library of Science (PLoS)
• Subjects: Algorithms; Amplitudes; Analysis; Engineering and Technology; Holography; Image reconstruction; Innovations; Java; Libraries; Microscope and microscopy; Microscopy; Physical Sciences; Programming languages; Propagation; Python; Research and Analysis Methods; Robustness (mathematics); Technology application; United States
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0275818

pyDHM is an open-source Python library aimed at Digital Holographic Microscopy (DHM) applications. The pyDHM is a user-friendly library written in the robust programming language of Python that provides a set of numerical processing algorithms for reconstructing amplitude and phase images for a broad range of optical DHM configurations. The pyDHM implements phase-shifting approaches for in-line and slightly off-axis systems and enables phase compensation for telecentric and non-telecentric systems. In addition, pyDHM includes three propagation algorithms for numerical focusing complex amplitude distributions in DHM and digital holography (DH) setups. We have validated the library using numerical and experimental holograms.