Macrophage Migration Inhibitory Factor as a Chaperone Inhibiting Accumulation of Misfolded SOD1

• Published in: Neuron (Cambridge, Mass.) Vol. 86; no. 1; pp. 218 - 232
• Main Authors: Israelson, Adrian, Ditsworth, Dara, Sun, Shuying, Song, SungWon, Liang, Jason, Hruska-Plochan, Marian, McAlonis-Downes, Melissa, Abu-Hamad, Salah, Zoltsman, Guy, Shani, Tom, Maldonado, Marcus, Bui, Anh, Navarro, Michael, Zhou, Huilin, Marsala, Martin, Kaspar, Brian K., Da Cruz, Sandrine, Cleveland, Don W.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 08.04.2015; Elsevier Limited
• Subjects: Acid Phosphatase - genetics; Amyotrophic lateral sclerosis; Animals; Cell Differentiation - genetics; Cells, Cultured; Choline O-Acetyltransferase - genetics; Choline O-Acetyltransferase - metabolism; Endoplasmic Reticulum - metabolism; Grants; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Humans; Induced Pluripotent Stem Cells; Isoenzymes - genetics; Liver; Liver - metabolism; Liver - ultrastructure; Macrophage Migration-Inhibitory Factors - metabolism; Mice; Mice, Transgenic; Microscopy; Mitochondria; Mitochondria - metabolism; Motor Neurons - physiology; Mutation; Mutation - genetics; Nerve Tissue Proteins - metabolism; Nervous system; Neurons; Protein Folding; Protein Transport - genetics; Rats; Rats, Transgenic; Ribosomal Proteins - genetics; Ribosomal Proteins - metabolism; Rodents; Spinal cord; Spinal Cord - metabolism; Spinal Cord - ultrastructure; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Superoxide Dismutase-1; Tartrate-Resistant Acid Phosphatase
• ISSN: 0896-6273; 1097-4199
• DOI: 10.1016/j.neuron.2015.02.034

Mutations in superoxide dismutase (SOD1) cause amyotrophic lateral sclerosis (ALS), a neurodegenerative disease characterized by loss of motor neurons and accompanied by accumulation of misfolded SOD1 onto the cytoplasmic faces of intracellular organelles, including mitochondria and the endoplasmic reticulum (ER). Using inhibition of misfolded SOD1 deposition onto mitochondria as an assay, a chaperone activity abundant in nonneuronal tissues is now purified and identified to be the multifunctional macrophage migration inhibitory factor (MIF), whose activities include an ATP-independent protein folding chaperone. Purified MIF is shown to directly inhibit mutant SOD1 misfolding. Elevating MIF in neuronal cells suppresses accumulation of misfolded SOD1 and its association with mitochondria and the ER and extends survival of mutant SOD1-expressing motor neurons. Accumulated MIF protein is identified to be low in motor neurons, implicating correspondingly low chaperone activity as a component of vulnerability to mutant SOD1 misfolding and supporting therapies to enhance intracellular MIF chaperone activity. •A cytosolic chaperone inhibits ALS-causing mutant SOD1 binding to mitochondria and ER•An unbiased screen identifies inhibition by MIF of ALS-causing mutant SOD1 misfolding•Direct action of the ATP-independent chaperone activity of MIF reduces misfolded SOD1•Elevation of MIF levels extends survival of mutant SOD1-expressing motor neurons Israelson et al. identify MIF as a chaperone inhibiting misfolding of ALS-linked SOD1 mutant proteins. Elevating MIF suppresses mutant SOD1 association with intracellular organelles and extends survival of mutant SOD1-expressing motor neurons, supporting therapies to enhance intracellular MIF chaperone activity.