Acute L-Arginine supplementation does not increase nitric oxide production in healthy subjects

• Published in: Nutrition & metabolism Vol. 9; no. 1; pp. 54 - 459
• Main Authors: Alvares, Thiago Silveira, Conte-Junior, Carlos Adam, Silva, Joab Trajano, Paschoalin, Vânia Margaret Flosi
• Format: Journal Article
• Language: English
• Published: London BioMed Central 12.06.2012; BioMed Central Ltd; BMC
• Subjects: acute effects; Amino acids; Analysis; analysis of variance; Arginine; Asymmetric dimethylarginine; blood; blood sampling; Chromatography; Clinical Nutrition; corn starch; derivatization; detectors; Dietary supplements; fluorescence; High performance liquid chromatography; HPLC; Hypertension; males; Measurement; Medicine; Medicine & Public Health; Metabolic Diseases; Metabolic disorders; muscles; Nitrate; nitrate reductase; Nitrates; Nitric oxide; Nitrite; nitrites; oxygen; placebos; Plasma; Proteins; Symmetric dimethylarginine; vasodilation; Nitrate; Nitric oxide; Amino acids; Asymmetric dimethylarginine; Symmetric dimethylarginine; Nitrite; HPLC
• ISSN: 1743-7075; 1743-7075
• DOI: 10.1186/1743-7075-9-54

Dietary supplements containing L-arginine have been marketed with the purpose of increasing vasodilatation, and thus, blood and oxygen supply to the exercising muscle. The present study evaluated the acute effect of L-arginine supplementation on indicators of NO production, nitrite (NO 2 - ) + nitrate (NO 3 - ) (NOx), in healthy subjects. Plasma concentrations of asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) have also been addressed. Seventeen healthy males participated in a randomized, double-blind, placebo-controlled study. Blood samples were drawn from a left antecubital vein at baseline (T0). Afterwards, subjects were randomly submittedto 6 g of oral L-arginine supplementation (as L-arginine hydrochloride) or placebo (as corn starch); afterwards, the subjects remained at rest in supine position and blood samples were drawn again at 30 (T1), 60 (T2), 90 (T3) and 120 minutes (T4) after supplementation. To analyze NO production, NO 3 - was converted to NO 2 - by nitrate reductase, followed by the derivatization of NO 2 - with 2,3-diaminonaphthalene. NOx, ADMA and SDMA were analyzed using a high-performance liquid chromatography system and monitored with a fluorescence detector. Two-way ANOVA with repeated measures showed no significant changes in NOx concentrations on the L-arginine group as compared to placebo group at any of the fivetime points (T0: 17.6 ± 3.9 vs 14.6 ± 2.3 μmol/L; T1: 15.8 ± 2.4 vs 14.3 ± 1.7 μmol/L; T2: 16.8 ± 4.9 vs 13.7 ± 2.7 μmol/L; T3: 16.7 ± 3.9 vs 14.6 ± 2.1 μmol/L; T4: 15.1 ± 2.8 vs 13.5 ± 3.5 μmol/L). Furthermore, plasma levels of ADMA and SDMA were not statistically significant between the L-arginine and placebo groups at T0 (0.43 ± 0.19 vs 0.39 ± 0.15 μmol/L and 1.83 ± 1.13 vs 1.70 ± 0.62 μmol/L), respectively. In conclusion, acute L-arginine supplementation does not increase plasma concentration of NOx in healthy individuals with normal plasma concentrations of ADMA.