青花菜C3H型锌指蛋白基因BoCCCH2的克隆与表达

以青花菜为材料,在克隆C3H型锌指蛋白基因BoCCCH2的基础上,研究该基因在不同器官及霜霉菌和灰葡萄孢菌侵染叶片中的表达模式。测序结果表明,BoCCCH2没有内含子,编码区全长为1 740bp,编码579个氨基酸,推导的蛋白质具2个ANK结构域和2种CCCH锌指结构,锌指结构的类型分别为C—X8—C—X5—C—X3—H和C—X5—C—X4—C—X3—H.反转录聚合酶链反应表明：BoCCCH2在根、叶、花茎、嫩角果、花蕾和花中均有表达,其中在根中的表达量最高;经霜霉菌和灰葡萄孢菌侵染后,该基因表达量均有不同程度的增加,其中在霜霉菌侵染下,表达量在24h后开始增加,72h时下降,而在灰葡萄孢菌侵...

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Published in浙江大学学报（农业与生命科学版） Vol. 42; no. 2; pp. 143 - 149
Main Author 蒋明 刘青娥 章燕如 祝琦 龚秀 俞可可 周秀倩
Format Journal Article
LanguageChinese
Published 台州学院生命科学学院生态学省重点学科,浙江 椒江,318000%丽水学院生态学院,浙江 丽水,323000 2016
Subjects
C3H型锌指
克隆
灰葡萄孢菌
表达
霜霉菌
青花菜
C3H型锌指
克隆
C3H-type zinc finger
expression
Brassica oleracea var . italica
Hyaloperonospora parasitica
Botrytis cinerea
青花菜
霜霉菌
灰葡萄孢菌
表达
cloning
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ISSN1008-9209
DOI10.3785/j.issn.1008-9209.2015.04.161

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Summary:以青花菜为材料,在克隆C3H型锌指蛋白基因BoCCCH2的基础上,研究该基因在不同器官及霜霉菌和灰葡萄孢菌侵染叶片中的表达模式。测序结果表明,BoCCCH2没有内含子,编码区全长为1 740bp,编码579个氨基酸,推导的蛋白质具2个ANK结构域和2种CCCH锌指结构,锌指结构的类型分别为C—X8—C—X5—C—X3—H和C—X5—C—X4—C—X3—H.反转录聚合酶链反应表明：BoCCCH2在根、叶、花茎、嫩角果、花蕾和花中均有表达,其中在根中的表达量最高;经霜霉菌和灰葡萄孢菌侵染后,该基因表达量均有不同程度的增加,其中在霜霉菌侵染下,表达量在24h后开始增加,72h时下降,而在灰葡萄孢菌侵染下,6h时的表达量最大,12h时开始缓慢下降。聚类结果表明,BoCCCH2与其他十字花科植物的同源序列聚为一类,支持率达100%,而与豆科、大戟科和蔷薇科等植物的序列处于不同分支。对BoCCCH2基因的克隆和表达分析为该基因功能研究奠定了基础。
Bibliography:33-1247/S
Brassica oleracea var.italicais an important vegetable crop worldwide,and in China,Taizhou City of Zhejiang Province is one of the major broccoli production areas.Downy mildew and grey mold rot are two common fungal diseases caused by Hyaloperonospora parasitica and Botrytis cinerea,respectively.In recent years,broccoli production in Taizhou was frequently affected by these two fungal diseases,resulting in yield and quality loss.Broccoli germplasm resources resistance to disease is scarce;therefore,molecular breeding is regarded as an effective solution to solve the problem.This is critically important to isolate genes associated with disease resistance,which will act as potential target genes for broccoli breeding.Zinc finger proteins are kinds of important transcription factors in eukaryotic organisms,which involve invarious biological activities,such as replication,transcription,translation,repair,metabolism and signaling.According to the number and order of cysteine and histidine residues,zinc fi
ISSN:1008-9209
DOI:10.3785/j.issn.1008-9209.2015.04.161