水稻低纤维突变体LCM527-1性状鉴定、遗传分析与基因定位

水稻茎秆机械强度是抗倒伏能力强弱的一项重要指标。为了深入研究水稻茎秆机械强度和抗倒伏特性的内在生理、生化、遗传和分子等方面机制,经400Gy ^60Coγ射线诱变和筛选,获得了一个特异性低纤维突变体LCM527-1。经考查,该突变体与野生型品种527（WT）相比,除整个生长期间表现出茎秆、叶片、叶鞘、穗颈和枝梗均脆性及极易折断外,还伴随有生长势偏弱、植株变矮、穗数减少、剑叶和穗变短等形态特性。抗倒伏指数、茎秆单位长度的鲜质量和干质量以及倒一节和倒二节抗折断力均明显降低,茎秆中Ca、Si、K等重要组成元素显著减少,茎秆纤维素含量显著下降,半纤维素含量增高。遗传分析表明,该低纤维突变性状受单隐性核...

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Published in浙江大学学报（农业与生命科学版） Vol. 41; no. 3; pp. 261 - 268
Main Author 童杰鹏 童川 王艳 任三娟 沈圣泉
Format Journal Article
LanguageChinese
Published 浙江大学原子核农业科学研究所,杭州,310029 2015
Subjects
低纤维
基因定位
抗倒伏性
水稻
突变体LCM527-1
遗传分析
突变体LCM527-1
遗传分析
genetic analysis
抗倒伏性
low cellulose mutant
基因定位
低纤维
gene mapping
水稻
LCM527-1
rice
lodging resistance
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ISSN1008-9209
DOI10.3785/j.issn.1008-9209.2014.11.041

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Summary:水稻茎秆机械强度是抗倒伏能力强弱的一项重要指标。为了深入研究水稻茎秆机械强度和抗倒伏特性的内在生理、生化、遗传和分子等方面机制,经400Gy ^60Coγ射线诱变和筛选,获得了一个特异性低纤维突变体LCM527-1。经考查,该突变体与野生型品种527（WT）相比,除整个生长期间表现出茎秆、叶片、叶鞘、穗颈和枝梗均脆性及极易折断外,还伴随有生长势偏弱、植株变矮、穗数减少、剑叶和穗变短等形态特性。抗倒伏指数、茎秆单位长度的鲜质量和干质量以及倒一节和倒二节抗折断力均明显降低,茎秆中Ca、Si、K等重要组成元素显著减少,茎秆纤维素含量显著下降,半纤维素含量增高。遗传分析表明,该低纤维突变性状受单隐性核基因控制（暂名为lcm527-1）,利用SSR分子标记将lcm527-1基因定位于2号染色体的RM3774和RM1092之间,遗传距离分别为10.6cM和5.1cM。这些结果将为今后研究低纤维突变及其精细定位提供依据,也为深入研究水稻抗倒伏机制提供分子生物学支持。
Bibliography:33-1247/S
Summary Low cellulose mutant in rice is a common type of crop culm mutants as reported in Arabidopsis,maize and barley previously.These mutants have obvious traits of fragility in stem and leaf,low mechanical strength and weaker breaking-resistance.A low cellulose mutant 527-1（LCM527-1）obtained from the offspring of a rice variety527 by 400Gy 60 Coγirradiation was applied to study it＇s biological phenotype,genetic traits and mutant gene,attempting to find reasons for low cellulose mutation and rice lodging.It exhibited fragility with continuity and integrity during the whole period of rice growth.In the process of experiment,some main investigations were made to identify the variation in biological expression,genetic analysis and gene mapping,concerning phenotype observation,agronomic traits,rice quality,chemical mineral elements,inheritance pattern and location of mutation gene.Four crosses were made to inspect the inheritance pattern and genetic stability between the low cellulose mutant527-1（femal
ISSN:1008-9209
DOI:10.3785/j.issn.1008-9209.2014.11.041