一种用于细胞实验的新型α粒子照射装置的建造和验证
整个装置由α粒子源、源支架和盖玻片盘等构成。根据理论推导可计算出样品中每个细胞接受到的α粒子数。利用Geant4程序包作为构建模型和模拟粒子输运的工具,还计算了盖玻片上细胞的剂量率分布,得出了盖玻片上离圆心不同距离处的剂量率分布图。最后,本研究还利用正常人皮肤成纤维细胞WS1,检测了用本照射仪照射细胞后30 min时的DNA双链断裂标记物-53BP1 foci的形成和照射后细胞的克隆生存曲线。结果显示,在盖玻片上不同位置处的细胞受照剂量存在差异,α粒子的剂量率与细胞与盖玻片圆心处的距离成反相关。与X-射线相比,α粒子造成的细胞死亡更严重,在SF为0.5时,其相对生物学效应RBE等于1.71。综...
Saved in:
| Published in | 辐射研究与辐射工艺学报 Vol. 33; no. 2; pp. 29 - 35 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
苏州大学医学部放射医学与防护学院/江苏高校放射医学协同创新中心 苏州215123
2015
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1000-3436 |
| DOI | 10.11889/j.1000-3436.2015.rrj.33.020205 |
Cover
| Abstract | 整个装置由α粒子源、源支架和盖玻片盘等构成。根据理论推导可计算出样品中每个细胞接受到的α粒子数。利用Geant4程序包作为构建模型和模拟粒子输运的工具,还计算了盖玻片上细胞的剂量率分布,得出了盖玻片上离圆心不同距离处的剂量率分布图。最后,本研究还利用正常人皮肤成纤维细胞WS1,检测了用本照射仪照射细胞后30 min时的DNA双链断裂标记物-53BP1 foci的形成和照射后细胞的克隆生存曲线。结果显示,在盖玻片上不同位置处的细胞受照剂量存在差异,α粒子的剂量率与细胞与盖玻片圆心处的距离成反相关。与X-射线相比,α粒子造成的细胞死亡更严重,在SF为0.5时,其相对生物学效应RBE等于1.71。综合以上结果,本研究设计并建造的α粒子照射仪是一个制作成本较低、使用方便、剂量精确的照射装置,满足放射生物学中在细胞及分子水平上研究α粒子的生物学效应的需要。 |
|---|---|
| AbstractList | 整个装置由α粒子源、源支架和盖玻片盘等构成。根据理论推导可计算出样品中每个细胞接受到的α粒子数。利用Geant4程序包作为构建模型和模拟粒子输运的工具,还计算了盖玻片上细胞的剂量率分布,得出了盖玻片上离圆心不同距离处的剂量率分布图。最后,本研究还利用正常人皮肤成纤维细胞WS1,检测了用本照射仪照射细胞后30 min时的DNA双链断裂标记物-53BP1 foci的形成和照射后细胞的克隆生存曲线。结果显示,在盖玻片上不同位置处的细胞受照剂量存在差异,α粒子的剂量率与细胞与盖玻片圆心处的距离成反相关。与X-射线相比,α粒子造成的细胞死亡更严重,在SF为0.5时,其相对生物学效应RBE等于1.71。综合以上结果,本研究设计并建造的α粒子照射仪是一个制作成本较低、使用方便、剂量精确的照射装置,满足放射生物学中在细胞及分子水平上研究α粒子的生物学效应的需要。 Q691.5%TL75+2.3; 整个装置由α粒子源、源支架和盖玻片盘等构成.根据理论推导可计算出样品中每个细胞接受到的α粒子数.利用Geant4程序包作为构建模型和模拟粒子输运的工具,还计算了盖玻片上细胞的剂量率分布,得出了盖玻片上离圆心不同距离处的剂量率分布图.最后,本研究还利用正常人皮肤成纤维细胞WS1,检测了用本照射仪照射细胞后30 min时的DNA双链断裂标记物-53BP1 foci的形成和照射后细胞的克隆生存曲线.结果显示,在盖玻片上不同位置处的细胞受照剂量存在差异,α粒子的剂量率与细胞与盖玻片圆心处的距离成反相关.与x-射线相比,α粒子造成的细胞死亡更严重,在SF为0.5时,其相对生物学效应RBE等于1.71.综合以上结果,本研究设计并建造的α粒子照射仪是一个制作成本较低、使用方便、剂量精确的照射装置,满足放射生物学中在细胞及分子水平上研究α粒子的生物学效应的需要. |
| Author | 嵇卫星 田文倩 尹晓明 杨红英 王敬东 |
| AuthorAffiliation | 苏州大学医学部放射医学与防护学院／江苏高校放射医学协同创新中心,苏州215123 |
| AuthorAffiliation_xml | – name: 苏州大学医学部放射医学与防护学院/江苏高校放射医学协同创新中心 苏州215123 |
| Author_FL | WANG Jingdong YIN Xiaoming YANG Hongying TIAN Wenqian JI Weixing |
| Author_FL_xml | – sequence: 1 fullname: JI Weixing – sequence: 2 fullname: TIAN Wenqian – sequence: 3 fullname: YIN Xiaoming – sequence: 4 fullname: YANG Hongying – sequence: 5 fullname: WANG Jingdong |
| Author_xml | – sequence: 1 fullname: 嵇卫星 田文倩 尹晓明 杨红英 王敬东 |
| BookMark | eNo9kDFLw0AYhm-oYK39Ey4uJt7lLne5UYpWoeDSvVySXm3QVBNEs1VsXawiRRwciuBQEazVSVr8C_6Kxmv_hScV-YZ3eJ_ve3m_JZAJG2EVgFUETYQch68HJoIQGphgaloQ2WYUBSbGJrT02BmQ_bcXQT6O6y60EWY2p1YWFCcfTdW_UrdPk9G1Gl9Mz3vpoDd77qj71vfdMO1dfr2p9276cqPa_XTYmj621edAm-l4NGs-pN2OZqevZ8tgQYr9uJr_0xwob22WC9tGabe4U9goGR4l1MCY-8izqoh6hFFOiCSEWMihLmc-k9BmlqREuJwL12eMUuRL6TsICeRTIQnOgbX52RMRShHWKkHjOAp1YEXGSZDIuJacur8_0M0h1fjKHPf2GmHtqK4XDqP6gYiSCqUE62zbwT9Rbng3 |
| ClassificationCodes | Q691.5%TL75+2.3 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP W92 ~WA 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.11889/j.1000-3436.2015.rrj.33.020205 |
| DatabaseName | 中文科技期刊数据库 中文科技期刊数据库-CALIS站点 维普中文期刊数据库 中文科技期刊数据库-工程技术 中文科技期刊数据库- 镜像站点 Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| DocumentTitleAlternate | The building and validation of a novel α-irradiation equipment used for cultured cell study |
| DocumentTitle_FL | The building and validation of a novel a-irradiation equipment used for cultured cell study |
| EndPage | 35 |
| ExternalDocumentID | fsyjyfsgyxb201502006 664344258 |
| GrantInformation_xml | – fundername: 国家自然科学基金; 江苏省教育厅省属高校自然科学研究重大项目; 江苏高校优势学科建设工程资助项目 funderid: (31270898、11335011); (12KJA310005); 江苏高校优势学科建设工程资助项目 |
| GroupedDBID | -03 2B. 2C0 2RA 5XA 5XD 92H 92I 92L ACGFS ALMA_UNASSIGNED_HOLDINGS CCEZO CEKLB CQIGP CW9 GROUPED_DOAJ TCJ TGT U1G U5M W92 ~WA 4A8 93N ABJNI PSX |
| ID | FETCH-LOGICAL-c646-339d1c2e16c476944f4442186b97d7f0572f64ab99abd77661dffd811a1d6af43 |
| ISSN | 1000-3436 |
| IngestDate | Thu May 29 04:11:10 EDT 2025 Wed Feb 14 10:30:44 EST 2024 |
| IsPeerReviewed | false |
| IsScholarly | true |
| Issue | 2 |
| Keywords | α-irradiation equipment 剂量率 克隆形成 Colony formation 53BP1 foci 241Am source Dose rate 241Am面源 α粒子照射装置 |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c646-339d1c2e16c476944f4442186b97d7f0572f64ab99abd77661dffd811a1d6af43 |
| Notes | JI Weixing ,TIAN Wenqian, YIN Xiaoming ,YANG Hongying ,WANG Jingdong （School of Radiation Medicine and Protection, Medical College of Soochow University / Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou 215123, China） 31-1258/TL α-irradiation equipment; 241Am source; Dose rate; 53BP1 foci; Colony formation The aim is to build a user-friendly α-irradiation equipment used for cultured cell study, and to validate its dosimetry and biological efficiency as well. The equipment is composed of α-particle source（241Am）, a holder for 241 Am, a holder plate for coverslips on which cells grow, and other essential parts. The theoretical average α-particle traversal per cell was calculated based on ideal assumptions. The Geant4 software package was used for modeling and simulating particle traversal. And the dose rate distribution in cells on coverslip was calculated and plotted versus the distance from the center of coverslip. Moreover, the induction of 53BP1 foci, a su |
| PageCount | 7 |
| ParticipantIDs | wanfang_journals_fsyjyfsgyxb201502006 chongqing_primary_664344258 |
| PublicationCentury | 2000 |
| PublicationDate | 2015 |
| PublicationDateYYYYMMDD | 2015-01-01 |
| PublicationDate_xml | – year: 2015 text: 2015 |
| PublicationDecade | 2010 |
| PublicationTitle | 辐射研究与辐射工艺学报 |
| PublicationTitleAlternate | Journal of Radiation Research and Radiation Processing |
| PublicationTitle_FL | Journal of Radiation Research and Radiation Processing |
| PublicationYear | 2015 |
| Publisher | 苏州大学医学部放射医学与防护学院/江苏高校放射医学协同创新中心 苏州215123 |
| Publisher_xml | – name: 苏州大学医学部放射医学与防护学院/江苏高校放射医学协同创新中心 苏州215123 |
| SSID | ssib051375962 ssib001129923 ssj0040334 ssib017479350 |
| Score | 2.0071607 |
| Snippet | 整个装置由α粒子源、源支架和盖玻片盘等构成。根据理论推导可计算出样品中每个细胞接受到的α粒子数。利用Geant4程序包作为构建模型和模拟粒子输运的工具,还计算了盖玻片上细... Q691.5%TL75+2.3; 整个装置由α粒子源、源支架和盖玻片盘等构成.根据理论推导可计算出样品中每个细胞接受到的α粒子数.利用Geant4程序包作为构建模型和模拟粒子输运的工具,还... |
| SourceID | wanfang chongqing |
| SourceType | Aggregation Database Publisher |
| StartPage | 29 |
| SubjectTerms | 241Am面源 53BP1 foci α粒子照射装置 克隆形成 剂量率 |
| Title | 一种用于细胞实验的新型α粒子照射装置的建造和验证 |
| URI | http://lib.cqvip.com/qk/90700X/201502/664344258.html https://d.wanfangdata.com.cn/periodical/fsyjyfsgyxb201502006 |
| Volume | 33 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals issn: 1000-3436 databaseCode: DOA dateStart: 20120101 customDbUrl: isFulltext: true dateEnd: 99991231 titleUrlDefault: https://www.doaj.org/ omitProxy: true ssIdentifier: ssj0040334 providerName: Directory of Open Access Journals |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Na9RAFA-lQvEifmKtSg8OHiTrJjOZzDsm3axFqKcKvS3JZtPSw1b7Abaniq0IVpEiHjwUwUNFsFZP0uK_4F_RNe1_4XuTZDe1RVovy_DmN--9-b3ZzAfzYRi3eFWBCGNl0rs0pkiqYIZOyzUjbC2xxSMFLh0UHnsgRx-K-xPORF__i9KupYX5qNJcOvZcyf9EFWUYVzole4rIdpWiANMYX_zFCOPviWLMAsF8RZsVApd5LlM1SoBgntJZHlMBSXyfKckCRHIGKHGYF-gEMA8xI7oUJgQLJAPJ_CphEKB8NhIw39JKbAa2LltjoC0qh4yiBPFUVjGPk5DANTLRU-uQD-gPWkRvvUy_rU13fcDidZa9rFIMl0noB9pc14pLxSFLAPNlQUJwHBgTCHN03UE7oP33JNVUeZhVtDiNdZjSFUIiPV-ToRjUexBNLrGjacqxWB84pKXKfNAQYMBzLSroQVBQ0yFyySPP1t4hPVZ5CSY7fqr_Lnm-quf1yUMocvbz-jhEIrLckwDFm-xI7XZwiJe_wV0SgdzFYOcEiUMKsUoe3aVLudgsoF7yDYgz0OYwQp51Mrua7KxqGDlqByhB1v1SW9S-IZ70YBPhd44yoseUvNTF0l0GXGTX3hR9MOelb41d7lChNDTLLrY52ukrBVmvX6imLZtOZXZ2usJ5BSdEdtXpjXe6u1CTucXpxWRucvFJRPhqdnf_GdvFMWtpcUZPLHBYXFpocCzu0rNVxZhPVHm2f6VwYMC4XXh2999-0Z0uUzPtycc4UtUHB9tJ2J4sjXHHzxvn8snpsJd9aS4YfUtTF42BsXz7zSXj3t6P5XTzVfr2097O63T3-f6zjc7WxsHntfT9yu93252Nl7--pd_XO1_epKubne2V_Y-r6c8tzOzs7hwsf-isryF2_-vTy8Z4PRgfGTXzl1jMphTS5Bxiq2m3LNkUrgQhEiEEPWYXgRu7CU757ESKMAIIo9gl-uIkiZVlhVYsw0TwK0Z_e6bdumoMK3rgvikcq0V6cH4iIyEAWkmCc4UkDAeNoS4djUfZhTsNidMmNOioQYPlBDXyz_Bc47goXjshbsg4S-lsSfW60T8_u9C6gZOM-eimjv8fOX_aLQ |
| linkProvider | Directory of Open Access Journals |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E4%B8%80%E7%A7%8D%E7%94%A8%E4%BA%8E%E7%BB%86%E8%83%9E%E5%AE%9E%E9%AA%8C%E7%9A%84%E6%96%B0%E5%9E%8B%CE%B1%E7%B2%92%E5%AD%90%E7%85%A7%E5%B0%84%E8%A3%85%E7%BD%AE%E7%9A%84%E5%BB%BA%E9%80%A0%E5%92%8C%E9%AA%8C%E8%AF%81&rft.jtitle=%E8%BE%90%E5%B0%84%E7%A0%94%E7%A9%B6%E4%B8%8E%E8%BE%90%E5%B0%84%E5%B7%A5%E8%89%BA%E5%AD%A6%E6%8A%A5&rft.au=%E5%B5%87%E5%8D%AB%E6%98%9F&rft.au=%E7%94%B0%E6%96%87%E5%80%A9&rft.au=%E5%B0%B9%E6%99%93%E6%98%8E&rft.au=%E6%9D%A8%E7%BA%A2%E8%8B%B1&rft.date=2015&rft.pub=%E8%8B%8F%E5%B7%9E%E5%A4%A7%E5%AD%A6%E5%8C%BB%E5%AD%A6%E9%83%A8%E6%94%BE%E5%B0%84%E5%8C%BB%E5%AD%A6%E4%B8%8E%E9%98%B2%E6%8A%A4%E5%AD%A6%E9%99%A2%2F%E6%B1%9F%E8%8B%8F%E9%AB%98%E6%A0%A1%E6%94%BE%E5%B0%84%E5%8C%BB%E5%AD%A6%E5%8D%8F%E5%90%8C%E5%88%9B%E6%96%B0%E4%B8%AD%E5%BF%83+%E8%8B%8F%E5%B7%9E215123&rft.issn=1000-3436&rft.volume=33&rft.issue=2&rft.spage=29&rft.epage=35&rft_id=info:doi/10.11889%2Fj.1000-3436.2015.rrj.33.020205&rft.externalDocID=fsyjyfsgyxb201502006 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F90700X%2F90700X.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Ffsyjyfsgyxb%2Ffsyjyfsgyxb.jpg |