一种用于细胞实验的新型α粒子照射装置的建造和验证

整个装置由α粒子源、源支架和盖玻片盘等构成。根据理论推导可计算出样品中每个细胞接受到的α粒子数。利用Geant4程序包作为构建模型和模拟粒子输运的工具,还计算了盖玻片上细胞的剂量率分布,得出了盖玻片上离圆心不同距离处的剂量率分布图。最后,本研究还利用正常人皮肤成纤维细胞WS1,检测了用本照射仪照射细胞后30 min时的DNA双链断裂标记物-53BP1 foci的形成和照射后细胞的克隆生存曲线。结果显示,在盖玻片上不同位置处的细胞受照剂量存在差异,α粒子的剂量率与细胞与盖玻片圆心处的距离成反相关。与X-射线相比,α粒子造成的细胞死亡更严重,在SF为0.5时,其相对生物学效应RBE等于1.71。综...

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Published in辐射研究与辐射工艺学报 Vol. 33; no. 2; pp. 29 - 35
Main Author 嵇卫星 田文倩 尹晓明 杨红英 王敬东
Format Journal Article
LanguageChinese
Published 苏州大学医学部放射医学与防护学院/江苏高校放射医学协同创新中心 苏州215123 2015
Subjects
241Am面源
53BP1
foci
α粒子照射装置
克隆形成
剂量率
α-irradiation equipment
剂量率
克隆形成
Colony formation
53BP1 foci
241Am source
Dose rate
241Am面源
α粒子照射装置
Online AccessGet full text
ISSN1000-3436
DOI10.11889/j.1000-3436.2015.rrj.33.020205

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Summary:整个装置由α粒子源、源支架和盖玻片盘等构成。根据理论推导可计算出样品中每个细胞接受到的α粒子数。利用Geant4程序包作为构建模型和模拟粒子输运的工具,还计算了盖玻片上细胞的剂量率分布,得出了盖玻片上离圆心不同距离处的剂量率分布图。最后,本研究还利用正常人皮肤成纤维细胞WS1,检测了用本照射仪照射细胞后30 min时的DNA双链断裂标记物-53BP1 foci的形成和照射后细胞的克隆生存曲线。结果显示,在盖玻片上不同位置处的细胞受照剂量存在差异,α粒子的剂量率与细胞与盖玻片圆心处的距离成反相关。与X-射线相比,α粒子造成的细胞死亡更严重,在SF为0.5时,其相对生物学效应RBE等于1.71。综合以上结果,本研究设计并建造的α粒子照射仪是一个制作成本较低、使用方便、剂量精确的照射装置,满足放射生物学中在细胞及分子水平上研究α粒子的生物学效应的需要。
Bibliography:JI Weixing ,TIAN Wenqian, YIN Xiaoming ,YANG Hongying ,WANG Jingdong （School of Radiation Medicine and Protection, Medical College of Soochow University / Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou 215123, China）
31-1258/TL
α-irradiation equipment; 241Am source; Dose rate; 53BP1 foci; Colony formation
The aim is to build a user-friendly α-irradiation equipment used for cultured cell study, and to validate its dosimetry and biological efficiency as well. The equipment is composed of α-particle source（241Am）, a holder for 241 Am, a holder plate for coverslips on which cells grow, and other essential parts. The theoretical average α-particle traversal per cell was calculated based on ideal assumptions. The Geant4 software package was used for modeling and simulating particle traversal. And the dose rate distribution in cells on coverslip was calculated and plotted versus the distance from the center of coverslip. Moreover, the induction of 53BP1 foci, a su
ISSN:1000-3436
DOI:10.11889/j.1000-3436.2015.rrj.33.020205