拟松材线虫携带细菌对云南松的致病性

为探讨拟松材线虫(Bursaphelenchus mucronatus)携带细菌在对云南松(Pinus yunnanensis)致病过程中的作用,选用二年生云南松苗木、从四川省松材线虫病病区分离到的拟松材线虫、线虫携带的细菌及其代谢产物为材料,通过室内对云南松枝条韧皮部滴管和松针浸渍法接种,观察并计算松针枯黄率,测定不同处理时间后松针的总酚含量和苯丙氨酸解氨酶(phenylalanine ammonia-lyase,PAL)、多酚氧化酶(polyphenol oxidase,PPO)、过氧化物酶(peroxidase,POD)等的酶活性变化来表征细菌及其代谢产物对云南松的致病力.结果表明,从拟...

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Published in浙江大学学报(农业与生命科学版) Vol. 40; no. 2; pp. 146 - 154
Main Author 李姝江 朱天辉 张兴华
Format Journal Article
LanguageChinese
Published 金堂县林业园林管理局,四川 金堂 610400 2014
四川农业大学林学院,四川 雅安,625014%四川农业大学林学院,四川 雅安 625014
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ISSN1008-9209
DOI10.3785/j.issn.1008-9209.2013.09.111

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Summary:为探讨拟松材线虫(Bursaphelenchus mucronatus)携带细菌在对云南松(Pinus yunnanensis)致病过程中的作用,选用二年生云南松苗木、从四川省松材线虫病病区分离到的拟松材线虫、线虫携带的细菌及其代谢产物为材料,通过室内对云南松枝条韧皮部滴管和松针浸渍法接种,观察并计算松针枯黄率,测定不同处理时间后松针的总酚含量和苯丙氨酸解氨酶(phenylalanine ammonia-lyase,PAL)、多酚氧化酶(polyphenol oxidase,PPO)、过氧化物酶(peroxidase,POD)等的酶活性变化来表征细菌及其代谢产物对云南松的致病力.结果表明,从拟松材线虫体表分离的26种细菌中,枯草芽孢杆菌Ⅲ(Bacillus subtilis Ⅲ)、嗜麦芽寡养单胞菌Ⅰ(Stenotrophomonas maltophilia Ⅰ)、杓兰果胶杆菌(Pectobacterium cypripedii)、解淀粉芽孢杆菌Ⅰ(B.amyloliquefaciens Ⅰ)、枯草芽孢杆菌Ⅴ(B.subtilis Ⅴ)所致段斑长度属于最高值范围且差异均无统计学意义.在进一步盆栽试验中仅杓兰果胶杆菌致病作用明显,其中,无菌拟松材线虫+杓兰果胶杆菌和无菌拟松材线虫+杓兰果胶杆菌非蛋白代谢产物的混合处理对云南松针叶致病性最强,但两者间差异无统计学意义,而与其余各处理间差异有统计学意义;在对云南松松针苯丙烷类代谢的影响中,灭菌线虫+杓兰果胶杆菌非蛋白代谢产物的混合处理作用最强,可以导致松针总酚含量,酚类抗氧化产物 POD、PPO、PAL 活性不断减少,且各指标与枯黄率显著相关.由此可知,云南松拟松材线虫病的发生、发展与寄主苯丙烷类代谢的异常变化有关.
Bibliography:33-1247/S
Li Shujiang , Zhu Tianhui, Zhang Xinghua ( 1. College of Forestry, Sichuan Agricultural University, Ya 'an, Sichuan 625014, China; 2. Jintang County Administration Bureau of Forestry and Gardens, Jintang, Sichuan 610400, China)
Bursaphelenchus mucronatus;bacterium;Pinus yunnanensis;pathogenicity
Summary Pine wood nematode (PWN) is a destructive disease to pines.For the research on its pathogenic mechanism,the theory which is about the complex infection of PWN and the bacterium carried by it is recognized. Bursaphelenchus mucronatus is considered as a related species of Bursaphelenchus xylophilus ,which may be differentiation with the homologous species in different environments.In early studies,B.mucronatus was served as contrast species to B.xylophilus due to the weak or lacking pathogenicity.But recently,many reports have pointed out that it has the strong pathogenicity and can cause the widespread death of pines,which referred to Pinus thunbergii P.yunnanensis P.taiwanensis P.densata ,etc.T
ISSN:1008-9209
DOI:10.3785/j.issn.1008-9209.2013.09.111