安多霖对高功率微波辐照大鼠睾丸生精细胞Caspase-9和XIAP的影响

为探讨安多霖对微波辐射损伤的的防治机制，将SD雄性大鼠按质量随机分为正常对照组、辐照组和预防组（给药量分别为3、6、9mg／kg）。预防组大鼠连续灌胃给药，对照组和辐照组给予双蒸水，20d后辐照组和预防组大鼠行100mw／cm2的高功率微波（nighpower-microwave，HPM、全身辐照10min。于辐照后第1、2、5天取睾丸组织制备病理标本，采用sP免疫组化检测试剂盒标定目的蛋白，Image．proplus图像分析软件对特定染色区域进行灰度分析和测定。结果显示，辐照组大鼠睾丸细胞Caspase．9水平较对照组呈“高-低-高”的“倒抛物线”形（p〈0．05），给药组则随着时间呈现“先...

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Published in辐射研究与辐射工艺学报 Vol. 34; no. 6; pp. 15 - 20
Main Author 郝述霞 吕慧敏 王春燕 齐雪松 佟鹏 苟巧
Format Journal Article
LanguageChinese
Published 中国疾病预防控制中心辐射防护与核安全医学所 北京 100088 2016
Subjects
Caspase-9
X连锁凋亡抑制蛋白（XIAP）
凋亡
安多霖
高功率微波
High power microwave
Anduolin
高功率微波
安多霖
Caspase-9
X连锁凋亡抑制蛋白(XIAP)
凋亡
X-linked inhibitor of apoptosis protein (XIAP)
Apoptosis
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ISSN1000-3436
DOI10.11889/j.1000-3436.2016.rrj.34.060201

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Summary:为探讨安多霖对微波辐射损伤的的防治机制，将SD雄性大鼠按质量随机分为正常对照组、辐照组和预防组（给药量分别为3、6、9mg／kg）。预防组大鼠连续灌胃给药，对照组和辐照组给予双蒸水，20d后辐照组和预防组大鼠行100mw／cm2的高功率微波（nighpower-microwave，HPM、全身辐照10min。于辐照后第1、2、5天取睾丸组织制备病理标本，采用sP免疫组化检测试剂盒标定目的蛋白，Image．proplus图像分析软件对特定染色区域进行灰度分析和测定。结果显示，辐照组大鼠睾丸细胞Caspase．9水平较对照组呈“高-低-高”的“倒抛物线”形（p〈0．05），给药组则随着时间呈现“先低后高”的趋势（p〈0．05）；对于x连锁凋亡抑制蛋白（x．1inkedinhibitorofapoptosisprotein，XIAP）而言，辐照组与对照组之间的差异不显著，而6、9mg/kg组在辐照后第5天较辐照组显著升高（p〈0．05）。结果提示，Caspase-9参与了HPM致大鼠睾丸细胞凋亡的过程，安多霖对大鼠睾丸细胞的保护作用需要Caspase-9和XIAP的共同参与。
Bibliography:High power microwave, Anduolin, Apoptosis, Caspase-9, X-linked inhibitor of apoptosis protein（XIAP）
HAO Shuxia, LYU Huimin ,WANG Chunyan, QI Xuesong, TONG Peng, GOU Qiao （National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beo＇ing 100088, China）
31-1258/TL
In order to investigate the Anduolin injury prevention mechanism of microwave exposure, sexually matured SD rats were randomly divided into control group, exposure group, and prevention groups （drug dosage were 3, 6 and 9 mg/kg）. Prevention groups were given drug continuously by oral administration for 20 d, meanwhile the control group and exposure group were given distilled water. After that the prevention groups and exposure group were exposed to high power-microwave （HPM） of 100 mW/cm2 for 10 min. Rats were killed at 1st, 2na, and 5th day after exposure, and the pathological specimens were prepared from testicular tissue. Interested proteins in testicular tissue were detected with immunohistochemical method, an
ISSN:1000-3436
DOI:10.11889/j.1000-3436.2016.rrj.34.060201