柑橘黄龙病菌亚洲种外膜蛋白基因的遗传变异分析

为了解柑橘黄龙病菌亚洲种（Candidatus Liberibacter asiaticus）的遗传多样性，对来源于国内外7个不同地区和浙江台州地区11个不同寄主品种的 Ca.L.asiaticus 的外膜蛋白（outer membrane protein，OMP）基因进行聚合酶链反应限制性片段长度多态性分析（polymerase chain reaction-restriction fragment length polymorphism，PCR-RFLP）.PCR 产物经克隆测序后，利用 NCBI Blast 和 DNAMAN 软件对 omp 基因序列进行多重比对，并用 MEGA 软件构建...

Full description

Saved in:
Bibliographic Details
Published in浙江大学学报（农业与生命科学版） Vol. 40; no. 2; pp. 125 - 132
Main Author 鹿连明 杜丹超 程保平 胡秀荣 张利平 陈国庆
Format Journal Article
LanguageChinese
Published 浙江省柑桔研究所,浙江 台州,318026%广东省农业科学院植物保护研究所,广州,510640 2014
Subjects
外膜蛋白
柑橘黄龙病菌亚洲种
遗传变异
限制性片段长度多态性分析
Candidatus Liberibacter asiaticus
遗传变异
genetic variation
限制性片段长度多态性分析
柑橘黄龙病菌亚洲种
restriction fragment length polymorphism analysis
外膜蛋白
outer membrane protein
Online AccessGet full text
ISSN1008-9209
DOI10.3785/j.issn.1008-9209.2013.05.241

Cover

More Information
Summary:为了解柑橘黄龙病菌亚洲种（Candidatus Liberibacter asiaticus）的遗传多样性，对来源于国内外7个不同地区和浙江台州地区11个不同寄主品种的 Ca.L.asiaticus 的外膜蛋白（outer membrane protein，OMP）基因进行聚合酶链反应限制性片段长度多态性分析（polymerase chain reaction-restriction fragment length polymorphism，PCR-RFLP）.PCR 产物经克隆测序后，利用 NCBI Blast 和 DNAMAN 软件对 omp 基因序列进行多重比对，并用 MEGA 软件构建系统发育树.结果显示：在浙江台州地区不同寄主品种上 Ca.L.asiaticus 各分离物的 omp 基因的酶切片段长度不具有多态性，其基因序列也完全一致；而不同地理来源的 Ca.L.asiaticus 的omp 基因经限制性内切酶消化后可产生不同的 RFLP 指纹图谱，其核酸序列和氨基酸序列均存在变异，表明 Ca. L.asiaticus 存在种内遗传多样性.在系统发育树上，所有的 Ca.L.asiaticus 分离物聚为1个分支，而 Ca.L. africanus 和 Ca.L.solanacearum 分别处于不同的分支上.
Bibliography:Summary Huanglongbing （HLB） is a destructive disease that represents a major threat to the world citrus industry.It was first reported from Chaoshan area of Guangdong Province in the early 20th century,and is now known to occur in next to 40 different Asian,African,Oceanian,South and North American countries and areas. Its pathogen is a phloem-limited,non-cultured,Gram-negative bacterium which belongs to the genus Candidatus Liberibacter.Three species,i.e.,Ca. L.asiaticus,Ca. L.americanus,and Ca. L.africanus with Ca. L. africanus subsp.capensis are currently known.So far,the pathogen of HLB disease discovered in China is only Ca.L.asiaticus,which distributes widely in almost all major citrus growing areas such as Guangdong,Guangxi, Fujian,Yunnan,Zhejiang and so on.This pathogen can be transmitted by grafting and Diaphorina citri and infect almost all fruit trees of Citrus Fortunella and Poncirus ,causing different characteristic symptoms including＆amp;nbsp;asymmetric blotchy mottle,vein yellowing or nutrition
ISSN:1008-9209
DOI:10.3785/j.issn.1008-9209.2013.05.241