虹鳟Ndufb2基因全长cDNA序列的克隆与分析

从虹鳟鱼（Oncorh ynchus mykiss）的脑组织中提取RNA，经逆转录一聚合酶链反应及eDNA末端快速扩增技术克隆出Ndu f62基因全长eDNA序列（GenBank登录号：FJ534641），并对其序列进行分析．扩增结果表明：Ndufb2基因的eDNA序列全长899bp，其中5’端非翻译区长152bp，3’端非翻译区长441bp，开放阅读框长306bp，编码101个氨基酸；其N端前50个氨基酸为线粒体靶序列将翻译的虹鳟Ndufb2蛋白序列与大西洋鲑（Sahno slar）的Ndufb2蛋白序列比对发现，其同源性高达98％，且虹鳟Ndufb2蛋白序列与已报道的哺乳动物的Ndufb2...

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Bibliographic Details
Published in浙江大学学报（农业与生命科学版） Vol. 39; no. 6; pp. 600 - 606
Main Author 王家庆 边佳 李代宗 马爽 王亮 那广宁
Format Journal Article
LanguageChinese
Published 沈阳工学院生命工程学院,辽宁 抚顺,113122%沈阳农业大学科学技术学院,辽宁 抚顺,113122%河北农业大学海洋学院,河北 秦皇岛,066003 2013
Subjects
cDNA末端快速扩增
Ndufb2基因
虹鳟鱼
逆转-聚合酶链反应
逆转录
reverse transcription-polymerase chain reaction
rainbow trout
rapid amplification of cDNA ends
虹鳟鱼
Ndufb2基因
Ndufb2 gene
Oncorhynchus mykiss
cDNA末端快速扩增
聚合酶链反应
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ISSN1008-9209
DOI10.3785/j.issn.1008-9209.2013.05.121

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Summary:从虹鳟鱼（Oncorh ynchus mykiss）的脑组织中提取RNA，经逆转录一聚合酶链反应及eDNA末端快速扩增技术克隆出Ndu f62基因全长eDNA序列（GenBank登录号：FJ534641），并对其序列进行分析．扩增结果表明：Ndufb2基因的eDNA序列全长899bp，其中5’端非翻译区长152bp，3’端非翻译区长441bp，开放阅读框长306bp，编码101个氨基酸；其N端前50个氨基酸为线粒体靶序列将翻译的虹鳟Ndufb2蛋白序列与大西洋鲑（Sahno slar）的Ndufb2蛋白序列比对发现，其同源性高达98％，且虹鳟Ndufb2蛋白序列与已报道的哺乳动物的Ndufb2蛋白序列同源性均在55％以上．表明Ndu、，陀基因在进化过程中是高度保守的，推测其在线粒体呼吸链组成中发挥着重要作用．
Bibliography:33-1247/S
WANG Jiaqing , BIAN Jia , LI Daizong , MA Shuang , WANG Liang , NA Guangning （ 1. College of Life and Engineering, Shenyang Institute of Technology, Fushun, Liaoning 113122, China; 2. College of Science and Technology, Shenyang Agricultural University, Fushun, Liaoning 113122, China; 3. Ocean College, Agricultural University of Hebei, Qinhuangdao, Hebei 066003, China）
Rainbow trout belongs to Salmonidae aerobic fish, and it is necessary for high dissolved oxygen content of living water environment. If the dissolved oxygen content of living water is less than 5 mg/L, it will cause the increase of respiratory rate, which is the so called ＂aquaculture floating head＂ phenomenon. Because the fish lives in hypoxia environment and the 90o/oo oxygen consumption is in the mitochondria, the transmission mechanism in composition and electronic respiratory chain may be different from the terrestrial animal. At the mitochondrial inner membrane, electrons from NADH and succinate pass through the electron transport
ISSN:1008-9209
DOI:10.3785/j.issn.1008-9209.2013.05.121