Inhibition of CaV3.2 T-type calcium channels in peripheral sensory neurons contributes to analgesic properties of epipregnanolone

• Published in: Psychopharmacology Vol. 231; no. 17; pp. 3503 - 3515
• Main Authors: Ayoola, Christine, Hwang, Sung Mi, Hong, Sung Jun, Rose, Kirstin E., Boyd, Christopher, Bozic, Neda, Park, Ji-Yong, Osuru, Hari Prasad, DiGruccio, Michael R., Covey, Douglas F., Jevtovic-Todorovic, Vesna, Todorovic, Slobodan M.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.09.2014; Springer; Springer Nature B.V
• Subjects: Analgesics; Analgesics - pharmacology; Animals; Behavior, Animal - drug effects; Biomedical and Life Sciences; Biomedicine; Calcium; Calcium Channel Blockers - pharmacology; Calcium channels; Calcium Channels, T-Type - drug effects; Calcium Channels, T-Type - genetics; Dosage and administration; Drug interactions; Female; Health aspects; Identification and classification; Male; Mice; Mice, Knockout; Neurons; Neurosciences; Nociceptors - drug effects; Original Investigation; Pain management; Pain Measurement - drug effects; Patch-Clamp Techniques; Peripheral Nerves - drug effects; Pharmacology/Toxicology; Pregnanolone - pharmacology; Psychiatry; Psychopharmacology; Rats; Sensory Receptor Cells - drug effects; Sensory receptors; Steroids (Drugs); Low-voltage-activated; Pain; Dorsal root; Ca; Hyperalgesia
• ISSN: 0033-3158; 1432-2072; 1432-2072
• DOI: 10.1007/s00213-014-3588-0

Rationale T-type calcium channels (T-channels) play an important role in controlling excitability of nociceptors. We have previously shown that a synthetic series of 5β-reduced steroids induce a voltage-dependent blockade of T-currents in rat dorsal root ganglia (DRG) cells in vitro and induce potent analgesia to thermal stimuli in rats in vivo (Mol Pharmacol 66:1223–1235, 2004 ). Objectives Here, we investigated the effects of the endogenous 5β-reduced neuroactive steroid molecule, epipregnanolone [(3β,5β)-3-hydroxypregnan-20-one], on peripheral nociception. Methods We used acutely dissociated DRG cells in vitro from adult rats as well as in vivo pain studies in mice and rats to investigate the effects of epipregnanolone on DRG T-channels. Results We found that epipregnanolone reversibly blocked DRG T-currents with a half-maximal inhibitory concentration (IC 50 ) of 2 μM and stabilized the channel in the inactive state. However, sodium, potassium, and gamma-aminobutyric acid (GABA)-gated ionic currents were not sensitive to the blocking effects of epipregnanolone even at 10 μM. In ensuing in vivo studies, we found that intraplantar (i.pl.) injections of epipregnanolone directly into peripheral receptive fields reduced responses to nociceptive heat stimuli in rats in a dose-dependent fashion. Furthermore, i.pl. epipregnanolone injections effectively reduced responses to peripheral nociceptive thermal and mechanical stimuli in wild-type mice but had no effect on the responses of Ca V 3.2 knockout mice. Conclusions We conclude that the inhibition of peripheral Ca V 3.2 T-channels contributes to the potent analgesic effect of the endogenous steroid epipregnanolone.