小儿气管炎颗粒指纹图谱的建立及其主要成分的含量测定

目的：建立小儿气管炎颗粒的指纹图谱及其主要成分（苦杏仁苷、紫菀等）的含量测定方法。方法：色谱柱为Agilent ZORBAX Eclipse XDB-C18色谱柱（4.6 mm×250 mm,5μm）;流动相为乙腈-0.1%磷酸水溶液,梯度洗脱;检测波长为207 nm;柱温为30℃;进样量为10μl;流速为1 ml/min。采用国家药典委员会《中药色谱指纹图谱相似度评价系统（2004 A版）》自动处理。结果：高效液相色谱图中有20个共有峰,11批小儿气管炎颗粒样品的相似度均〉0.9。3批小儿气管炎颗粒样品中苦杏仁苷和紫菀酮含量分别为（18.74±0.03）和（17.58±0.04）μg/g。结...

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Bibliographic Details
Published in中国医院用药评价与分析 Vol. 17; no. 9; pp. 1180 - 1182
Main Author 张建春 李茜 张琛
Format Journal Article
LanguageChinese
Published 兰州大学药学院,甘肃 兰州 730000 2017
解放军第一医院药剂科,甘肃兰州 730000%兰州大学药学院,甘肃 兰州,730000%解放军第一医院药剂科,甘肃兰州,730000
Subjects
小儿气管炎颗粒
指纹图谱
高效液相色谱法
高效液相色谱法
小儿气管炎颗粒
Xiaoerqiguanyan granules
Fingerprints
HPLC
指纹图谱
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ISSN1672-2124
DOI10.14009/j.issn.1672-2124.2017.09.010

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Summary:目的：建立小儿气管炎颗粒的指纹图谱及其主要成分（苦杏仁苷、紫菀等）的含量测定方法。方法：色谱柱为Agilent ZORBAX Eclipse XDB-C18色谱柱（4.6 mm×250 mm,5μm）;流动相为乙腈-0.1%磷酸水溶液,梯度洗脱;检测波长为207 nm;柱温为30℃;进样量为10μl;流速为1 ml/min。采用国家药典委员会《中药色谱指纹图谱相似度评价系统（2004 A版）》自动处理。结果：高效液相色谱图中有20个共有峰,11批小儿气管炎颗粒样品的相似度均〉0.9。3批小儿气管炎颗粒样品中苦杏仁苷和紫菀酮含量分别为（18.74±0.03）和（17.58±0.04）μg/g。结论：该方法可作为小儿气管炎颗粒质控方法。
Bibliography:ZHANG Jianchun1,2, LI Qian1, ZHANG Chen2 （1. Pharmacy College, Lanzhou University, Gansu Lanzhou 730000, China; 2. Dept. of Pharmacy, the First Hospital of PLA, Gansu Lanzhou 730000, China）
Xiaoerqiguanyan granules ; Fingerprints ; HPLC
11-4975/R
OBJECTIVE： To built the method for establishment of fingerprint and main content（ amygdalin,tartarian aster） determination of Xiaoerqiguanyan granules. METHODS： The column was Agilent ZORBAX SB-C18（ 4. 6 mm × 250 mm,5 μm）,the mobile phase was acetonitrile-0. 1% phosphoric acid solution with gradient elution,the flow rate was 1 ml/min,and the detection wavelength was set at 207 nm with the column temperature of 30 ℃.The sample size was 10 μl with flow rate of 1 ml/min. Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine（ Version 2004A） published by GPC was adopt for the auto-processing. RESULTS： There were 20 common peaks in HPLC chromatograms,the similarity of 11 batches of Xiaoerqiguanyan granules were 0. 9.The content of amygda
ISSN:1672-2124
DOI:10.14009/j.issn.1672-2124.2017.09.010