高效液相色谱法同时测定头痛宁口服液中芍药苷和阿魏酸的含量

目的：建立同时测定头痛宁口服液中芍药苷和阿魏酸含量的高效液相色谱法。方法：色谱柱为Amethyst C18-H柱（200 mm×4.6 mm,5μm）,流动相为乙腈-0.1%磷酸（V∶V=14∶86）,流速1.0 ml/min,柱温30℃,芍药苷的检测波长为230 nm,阿魏酸为316 nm。结果：芍药苷进样量在0.312 3-3.123 0μg范围内线性关系良好（r=0.999 9）,平均加样回收率为99.88%（n=9,RSD=0.09%）;阿魏酸进样量在0.015 7-0.157 0μg范围内线性关系良好（r=1.000 0）,平均加样回收率为99.75%（n=9,RSD=0.16%）。...

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Bibliographic Details
Published in中国医院用药评价与分析 Vol. 17; no. 9; pp. 1232 - 1233
Main Author 张顺平 袁建平 吕艳
Format Journal Article
LanguageChinese
Published 曲靖市食品药品检验检测中心中药室,云南曲靖,655000%曲靖市第一人民医院药剂科,云南曲靖,655000 2017
Subjects
含量测定
头痛宁口服液
芍药苷
阿魏酸
高效液相色谱法
含量测定
Paeoniflorin
Toutongning oral liquid
高效液相色谱法
头痛宁口服液
Content determination
HPLC
芍药苷
阿魏酸
Ferulic acid
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ISSN1672-2124
DOI10.14009/j.issn.1672-2124.2017.09.030

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Summary:目的：建立同时测定头痛宁口服液中芍药苷和阿魏酸含量的高效液相色谱法。方法：色谱柱为Amethyst C18-H柱（200 mm×4.6 mm,5μm）,流动相为乙腈-0.1%磷酸（V∶V=14∶86）,流速1.0 ml/min,柱温30℃,芍药苷的检测波长为230 nm,阿魏酸为316 nm。结果：芍药苷进样量在0.312 3-3.123 0μg范围内线性关系良好（r=0.999 9）,平均加样回收率为99.88%（n=9,RSD=0.09%）;阿魏酸进样量在0.015 7-0.157 0μg范围内线性关系良好（r=1.000 0）,平均加样回收率为99.75%（n=9,RSD=0.16%）。结论：本方法简便、快捷,精密度、重复性好,可用于头痛宁口服液的质量控制。
Bibliography:Toutongning oral liquid ; Paeoniflorin ; Ferulic acid ; HPLC ; Content determination
ZHANG Shunping1, YUAN Jianping2 , LYU Yan2（ 1. Dept. of Chinese Medicine, Qujing Inspection Center for Food and Drug, Yunnan Qujing 655000, China; 2. Dept. of Pharmacy, the First People＇s Hospital of Qujing, Yunnan Qujing 655000,China）
OBJECTIVE： To establish the method for content determination of paeoniflorin and ferulic acid in Toutongning oral liquid by HPLC. METHODS： The column was Amethyst C18-H（ 200 mm × 4. 6 mm,5 μm）,the mobile phase consisted of acetonitrile-0. 1% phosphoric acid（ V ∶ V = 14 ∶ 86） at the flow rate of 1. 0 ml/min. The column temperature was 30 ℃,the detection wavelength of paeoniflorin and ferulic were respectively 230 nm and316 nm. RESULTS： Good linearity was shown in the range of 0. 312 3-3. 123 0 μg（ r = 0. 999 9） for paeoniflorin with the average recovery of 99. 88%（ n = 9,RSD = 0. 09%） and 0. 0157 0-0. 157 0 μg（ r = 1. 000 0） for ferulic acid with the average recovery of 99. 75%（ n = 9,RSD = 0. 16%
ISSN:1672-2124
DOI:10.14009/j.issn.1672-2124.2017.09.030