新型C18核壳结构色谱柱快速分析人参中的3种皂苷
目的建立高效液相色谱法（high performance liquid chromatography,HPLC）测定人参中人参皂苷Rg1、Re和Rb1 3种人参皂苷的含量。方法采用月旭Boltimate-（TM）C18新型核壳色谱柱对3种人参皂苷进行分离,以乙腈-1.0%磷酸水溶液为流动相进行梯度洗脱,采用HPLC进行检测。结果 3种人参皂苷在月旭Boltimate-（TM）C18新型核壳色谱柱能够快速分离,且分离效果较好。3种人参皂苷的在0.4-4.0μg质量浓度范围内呈良好的线性关系,线性相关系数（R^2）分别为0.9998,0.9996和0.9997;Rg1、Re和Rb1检出限分别为0....
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| Published in | 食品安全质量检测学报 Vol. 7; no. 7; pp. 2763 - 2769 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
浙江师范大学物理化学研究所,金华,321004%浙江师范大学物理化学研究所,金华321004
2016
浙江月旭材料科技有限公司,金华321014%浙江月旭材料科技有限公司,金华,321014 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-0381 |
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| Abstract | 目的建立高效液相色谱法（high performance liquid chromatography,HPLC）测定人参中人参皂苷Rg1、Re和Rb1 3种人参皂苷的含量。方法采用月旭Boltimate-（TM）C18新型核壳色谱柱对3种人参皂苷进行分离,以乙腈-1.0%磷酸水溶液为流动相进行梯度洗脱,采用HPLC进行检测。结果 3种人参皂苷在月旭Boltimate-（TM）C18新型核壳色谱柱能够快速分离,且分离效果较好。3种人参皂苷的在0.4-4.0μg质量浓度范围内呈良好的线性关系,线性相关系数（R^2）分别为0.9998,0.9996和0.9997;Rg1、Re和Rb1检出限分别为0.003、0.001和0.002μg/kg;加标回收率为98.3%-101.3%,相对标准偏差RSD分别为0.8%、1.1%和1.5%（n=6）。结论本方法具有快速、柱压低及节省溶剂等优势,可用于人参提取物中人参皂苷的测定。 |
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| AbstractList | 目的 建立高效液相色谱法(high performance liquid chromatography,HPLC)测定人参中人参皂苷Rg1、Re和Rb13种人参皂苷的含量.方法 采用月旭BoltimateTM C18新型核壳色谱柱对3种人参皂苷进行分离,以乙腈-1.0％磷酸水溶液为流动相进行梯度洗脱,采用HPLC进行检测.结果 3种人参皂苷在月旭BoltimateTMC18新型核壳色谱柱能够快速分离,且分离效果较好.3种人参皂苷的在0.4～4.0 μg质量浓度范围内呈良好的线性关系,线性相关系数(R2)分别为0.9998,0.9996和0.9997;Rg1、Re和Rb1检出限分别为0.003、0.001和0.002μg/kg;加标回收率为98.3％～101.3％,相对标准偏差RSD分别为0.8％、1.1％和1.5％(n=6).结论 本方法具有快速、柱压低及节省溶剂等优势,可用于人参提取物中人参皂苷的测定. 目的建立高效液相色谱法（high performance liquid chromatography,HPLC）测定人参中人参皂苷Rg1、Re和Rb1 3种人参皂苷的含量。方法采用月旭Boltimate-（TM）C18新型核壳色谱柱对3种人参皂苷进行分离,以乙腈-1.0%磷酸水溶液为流动相进行梯度洗脱,采用HPLC进行检测。结果 3种人参皂苷在月旭Boltimate-（TM）C18新型核壳色谱柱能够快速分离,且分离效果较好。3种人参皂苷的在0.4-4.0μg质量浓度范围内呈良好的线性关系,线性相关系数（R^2）分别为0.9998,0.9996和0.9997;Rg1、Re和Rb1检出限分别为0.003、0.001和0.002μg/kg;加标回收率为98.3%-101.3%,相对标准偏差RSD分别为0.8%、1.1%和1.5%（n=6）。结论本方法具有快速、柱压低及节省溶剂等优势,可用于人参提取物中人参皂苷的测定。 |
| Author | 岳焕新 薛昆鹏 金小青 李良翔 赵岳星 |
| AuthorAffiliation | 浙江师范大学物理化学研究所,金华321004 浙江月旭材料科技有限公司,金华321014 |
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| Author_FL | XUE Kun-Peng YUE Huan-Xin JIN Xiao-Qing LI Liang-Xiang ZHAO Yue-Xing |
| Author_FL_xml | – sequence: 1 fullname: YUE Huan-Xin – sequence: 2 fullname: XUE Kun-Peng – sequence: 3 fullname: JIN Xiao-Qing – sequence: 4 fullname: LI Liang-Xiang – sequence: 5 fullname: ZHAO Yue-Xing |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| Discipline | Diet & Clinical Nutrition |
| DocumentTitleAlternate | Rapid determination of 3 kinds of ginsenosides in Panax ginseng by new C18 core-shell column |
| DocumentTitle_FL | Rapid determination of 3 kinds of ginsenosides in Panax ginseng by new C18 core-shell column |
| EndPage | 2769 |
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| GrantInformation_xml | – fundername: 浙江省重大科技专项重点工业项目(2014C01025) Supported by Major Science and Technology Key Industrial Project of Zhejiang Province funderid: (2014C01025) |
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| Issue | 7 |
| Keywords | 人参 high performance liquid chromatography core-shell column ginsenoside 高效液相色谱 核壳色谱柱 Panax ginseng 人参皂苷 |
| Language | Chinese |
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| Notes | YUE Huan-Xin1, XUE Kun-Peng1,2, JIN Xiao-Qing1,2, LI Liang-Xiang2, ZHAO Yue-Xing1,2 （1. Zhejiang Normal University Institute of Physical Chemistry, Jinhua 321004, China 2. Welch Materials （Zhejiang） Co., Ltd., Jinhua 321014, China） Objective To establish a method for the determination of 3 kinds of ginsenosides including Rgl, Re and Rbl in Panax ginseng by high performance liquid chromatography （HPLC）. Methods Samples were separated by Welch BoltimateTM C18 core-shell column with the mobile phase of acetonitrile and phosphoric acid aqueous solution for gradient elution, and then detected by HPLC. Results Using Welch BoltimateTM C18 core-shell column, 3 kinds of ginsenosides could be separated rapidly and well. The calibration curves showed good linear relationships in the mass concentration range of 0.4-4.0 pg with correlation coefficients of 0.9998, 0.9996 and 0.9997, respectively The limits of detection （LODs） were 0.003, 0.001 and 0.002 9g/kg for Rgb Re and Rbl, respectively. The recoveries were 98.3%-101.3 |
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| PublicationDate | 2016 |
| PublicationDateYYYYMMDD | 2016-01-01 |
| PublicationDate_xml | – year: 2016 text: 2016 |
| PublicationDecade | 2010 |
| PublicationTitle | 食品安全质量检测学报 |
| PublicationTitleAlternate | Journal of Food Safety and Quality |
| PublicationTitle_FL | Journal of Food Safety & Quality |
| PublicationYear | 2016 |
| Publisher | 浙江师范大学物理化学研究所,金华,321004%浙江师范大学物理化学研究所,金华321004 浙江月旭材料科技有限公司,金华321014%浙江月旭材料科技有限公司,金华,321014 |
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| Snippet | 目的建立高效液相色谱法（high performance liquid chromatography,HPLC）测定人参中人参皂苷Rg1、Re和Rb1 3种人参皂苷的含量。方法采用月旭Boltimate-（TM）C18新型核壳色... 目的 建立高效液相色谱法(high performance liquid chromatography,HPLC)测定人参中人参皂苷Rg1、Re和Rb13种人参皂苷的含量.方法 采用月旭BoltimateTM C18新型核壳色谱柱... |
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| SubjectTerms | 人参 人参皂苷 核壳色谱柱 高效液相色谱 |
| Title | 新型C18核壳结构色谱柱快速分析人参中的3种皂苷 |
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