新型C18核壳结构色谱柱快速分析人参中的3种皂苷

目的建立高效液相色谱法（high performance liquid chromatography,HPLC）测定人参中人参皂苷Rg1、Re和Rb1 3种人参皂苷的含量。方法采用月旭Boltimate-（TM）C18新型核壳色谱柱对3种人参皂苷进行分离,以乙腈-1.0%磷酸水溶液为流动相进行梯度洗脱,采用HPLC进行检测。结果 3种人参皂苷在月旭Boltimate-（TM）C18新型核壳色谱柱能够快速分离,且分离效果较好。3种人参皂苷的在0.4-4.0μg质量浓度范围内呈良好的线性关系,线性相关系数（R^2）分别为0.9998,0.9996和0.9997;Rg1、Re和Rb1检出限分别为0....

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Bibliographic Details
Published in食品安全质量检测学报 Vol. 7; no. 7; pp. 2763 - 2769
Main Author 岳焕新 薛昆鹏 金小青 李良翔 赵岳星
Format Journal Article
LanguageChinese
Published 浙江师范大学物理化学研究所,金华,321004%浙江师范大学物理化学研究所,金华321004 2016
浙江月旭材料科技有限公司,金华321014%浙江月旭材料科技有限公司,金华,321014
Subjects
人参
人参皂苷
核壳色谱柱
高效液相色谱
人参
high performance liquid chromatography
core-shell column
ginsenoside
高效液相色谱
核壳色谱柱
Panax ginseng
人参皂苷
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ISSN2095-0381

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Summary:目的建立高效液相色谱法（high performance liquid chromatography,HPLC）测定人参中人参皂苷Rg1、Re和Rb1 3种人参皂苷的含量。方法采用月旭Boltimate-（TM）C18新型核壳色谱柱对3种人参皂苷进行分离,以乙腈-1.0%磷酸水溶液为流动相进行梯度洗脱,采用HPLC进行检测。结果 3种人参皂苷在月旭Boltimate-（TM）C18新型核壳色谱柱能够快速分离,且分离效果较好。3种人参皂苷的在0.4-4.0μg质量浓度范围内呈良好的线性关系,线性相关系数（R^2）分别为0.9998,0.9996和0.9997;Rg1、Re和Rb1检出限分别为0.003、0.001和0.002μg/kg;加标回收率为98.3%-101.3%,相对标准偏差RSD分别为0.8%、1.1%和1.5%（n=6）。结论本方法具有快速、柱压低及节省溶剂等优势,可用于人参提取物中人参皂苷的测定。
Bibliography:YUE Huan-Xin1, XUE Kun-Peng1,2, JIN Xiao-Qing1,2, LI Liang-Xiang2, ZHAO Yue-Xing1,2 （1. Zhejiang Normal University Institute of Physical Chemistry, Jinhua 321004, China 2. Welch Materials （Zhejiang） Co., Ltd., Jinhua 321014, China）
Objective To establish a method for the determination of 3 kinds of ginsenosides including Rgl, Re and Rbl in Panax ginseng by high performance liquid chromatography （HPLC）. Methods Samples were separated by Welch BoltimateTM C18 core-shell column with the mobile phase of acetonitrile and phosphoric acid aqueous solution for gradient elution, and then detected by HPLC. Results Using Welch BoltimateTM C18 core-shell column, 3 kinds of ginsenosides could be separated rapidly and well. The calibration curves showed good linear relationships in the mass concentration range of 0.4-4.0 pg with correlation coefficients of 0.9998, 0.9996 and 0.9997, respectively The limits of detection （LODs） were 0.003, 0.001 and 0.002 9g/kg for Rgb Re and Rbl, respectively. The recoveries were 98.3%-101.3
ISSN:2095-0381