Levels of follicular G-CSF and interleukin-15 appear as noninvasive biomarkers of subsequent successful birth in modified natural in vitro fertilization/intracytoplasmic sperm injection cycles

• Published in: Fertility and sterility Vol. 95; no. 1; pp. 94 - 98
• Main Authors: Lédée, Nathalie, Frydman, René, Osipova, Agnessia, Taieb, Joelle, Gallot, Vanessa, Lombardelli, Letizia, Logiodice, Federica, Petitbarat, Marie, Fanchin, Renato, Chaouat, Gérard, Achour-Frydman, Nelly, Piccinni, Marie Pierre
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 2011; Elsevier
• Subjects: Adult; Biological and medical sciences; biomarker; biomarkers; Biomarkers - metabolism; Birth control; chemokines; conception; embryo implantation; Female; follicular fluid; Follicular Fluid - metabolism; G-CSF; Granulocyte Colony-Stimulating Factor - metabolism; growth factors; Gynecology. Andrology. Obstetrics; Humans; immunoassays; interleukin-15; Interleukin-15 - metabolism; Internal Medicine; intracytoplasmic sperm injection; IVF/ICSI; Medical sciences; monitored natural cycle; Multivariate Analysis; Obstetrics and Gynecology; oocyte; oocytes; Ovarian Follicle - metabolism; patients; Predictive Value of Tests; Pregnancy; Pregnancy Outcome; Pregnancy Rate; Prognosis; regression analysis; Retrospective Studies; Sperm Injections, Intracytoplasmic; Sterility. Assisted procreation; women; interleukin-15; oocyte; monitored natural cycle; pregnancy; biomarker; G-CSF; follicular fluid; IVF/ICSI; Ovarian follicular fluid; Intracytoplasmic sperm injection; Gynecology; Biological marker; Assisted procreation; Interleukin 15; Birth; Obstetrics; In vitro fertilization embryo transfer; Pregnancy; Granulocyte colony stimulating factor; Oocyte
• ISSN: 0015-0282; 1556-5653; 1556-5653
• DOI: 10.1016/j.fertnstert.2010.03.010

To explore oocyte competence for subsequent birth. The modified natural IVF/intracytoplasmic sperm injection (ICSI) cycle was used as an experimental model by measuring levels of cytokines, chemokines, and growth factors in individual follicular fluids (FF). A retrospective blinded study. European network of research, Embryo Implantation Control (EMBIC). Single FF from 83 women were analyzed during a modified natural IVF/ICSI cycle, and reproducibility of follicular composition was evaluated over two cycles for 15 patients. Each FF sample was blindly tested to assess levels of 26 factors by bead-based immunoassays. Each mediator was evaluated as a potential biomarker of subsequent birth by multivariate regression analysis. A combination of both FF G-CSF and IL-15 was the optimal model to predict birth (AUC ROC, 0.85). Birth rates per cycle were 48.9% (16/33) if two good-prognosis criteria were present (FF G-CSF >12 pg/mL and IL-15 <7 pg/mL) and 8% (3/36) and 0% (0/14) if, respectively, one or none were present. FF G-CSF was significantly correlated over two cycles ( r = .71), suggesting a possible prognostic value of its documentation. Combined follicular G-CSF and IL-15 quantification appears as an efficient and noninvasive method to define oocyte competence for subsequent successful conception in modified natural IVF/ICSI cycles.