Genomic Evolution of MHC Class I Region in Primates

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 102; no. 26; pp. 9230 - 9234
• Main Authors: Fukami-Kobayashi, Kaoru, Shiina, Takashi, Anzai, Tatsuya, Sano, Kazumi, Yamazaki, Masaaki, Inoko, Hidetoshi, Tateno, Yoshio, Ohta, Tomoko
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 28.06.2005; National Acad Sciences
• Subjects: Animals; Apes; Biological Sciences; Chimpanzees; Divergent evolution; Evolution; Evolution, Molecular; Genes, MHC Class I - genetics; Genome; Genomes; Genomics; Histocompatibility Antigens Class I - genetics; HLA-B Antigens - genetics; HLA-C Antigens - genetics; Humans; Long Interspersed Nucleotide Elements; Macaca mulatta; Mica; Molecules; Monkeys; Pan troglodytes; Primates; Protein Structure, Tertiary; Regional identity; Time Factors
• ISSN: 0027-8424; 1091-6490; 1091-6490
• DOI: 10.1073/pnas.0500770102

To elucidate the origins of the MHC-B-MHC-C pair and the MHC class I chain-related molecule (MIC)A-MICB pair, we sequenced an MHC class I genomic region of humans, chimpanzees, and rhesus monkeys and analyzed the regions from an evolutionary standpoint, focusing first on LINE sequences that are paralogous within each of the first two species and orthologous between them. Because all the long interspersed nuclear element (LINE) sequences were fragmented and nonfunctional, they were suitable for conducting phylogenetic study and, in particular, for estimating evolutionary time. Our study has revealed that MHC-B and MHC-C duplicated 22.3 million years (Myr) ago, and the ape MICA and MICB duplicated 14.1 Myr ago. We then estimated the divergence time of the rhesus monkey by using other orthologous LINE sequences in the class I regions of the three primate species. The result indicates that rhesus monkeys, and possibly the Old World monkeys in general, diverged from humans 27-30 Myr ago. Interestingly, rhesus monkeys were found to have not the pair of MHC-B and MHC-C but many repeated genes similar to MHC-B. These results support our inference that MHC-B and MHC-C duplicated after the divergence between apes and Old World monkeys.