基于新型立体结构的色谱固定相的高效液相色谱法检测分离7种有机酸
目的制备新型立体结构色谱固定相并开发新的高效液相色谱法（high performance liquid chromatography,HPLC）分离7种有机酸的方法。方法采用新型立体结构固定相对7种有机酸进行分离，以5％甲醇．95％10mmol／L甲酸铵（pH=3．0）水溶液作为流动相进行等度洗脱；流速为1．0mL／min；柱温：40℃；紫外检测波长：220nm；进样体积：10uL，采用HPLC进行检测。结果制备了新型立体结构固定相，具有与常规c18固定相相似的疏水作用，8min内完全分离7种有机酸，且分离效果较好。结论本方法具有保留增强、快速及分离效果好等优势，可用于多种有机酸的分离测定。...
Saved in:
| Published in | 食品安全质量检测学报 Vol. 9; no. 1; pp. 167 - 172 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
浙江师范大学生化学院,金华,321004
2018
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-0381 |
Cover
| Summary: | 目的制备新型立体结构色谱固定相并开发新的高效液相色谱法（high performance liquid chromatography,HPLC）分离7种有机酸的方法。方法采用新型立体结构固定相对7种有机酸进行分离，以5％甲醇．95％10mmol／L甲酸铵（pH=3．0）水溶液作为流动相进行等度洗脱；流速为1．0mL／min；柱温：40℃；紫外检测波长：220nm；进样体积：10uL，采用HPLC进行检测。结果制备了新型立体结构固定相，具有与常规c18固定相相似的疏水作用，8min内完全分离7种有机酸，且分离效果较好。结论本方法具有保留增强、快速及分离效果好等优势，可用于多种有机酸的分离测定。 |
|---|---|
| Bibliography: | ZHANG Chuan, JIN Xiao-Qing, ZHAO Yue-Xing （Institute of Physical Chemistry, Zhejiang Normal University, Jinhua 321004, China） 11-5956/TS Objective To prepare a new solid structure stationary phase and establish a new method for separation of 7 organic acids by high performance liquid chromatography （HPLC）. Methods Totally 7 organic acids were separated by new solid structure stationary phase with the 5% methanol-95% 10 mmol/L ammonium formate （pH=3.0） solution as a mobile phase for isocratic elution. Flow rate was 1.0 mL/min, column temperature was 40 ℃, detection wavelength was 220 nm and injection volume was 10μL. Then the samples were detected by HPLC. Results A new solid structure stationary phase was prepared, which had hydrophobic effect similar to that of C18 stationary phase by new solid structure stationary phase. Totally 7 organic acids were completely separated within 8 min, and the separation effect was better. Conclusions The established method has the advantages of strong retention, rapid determin |
| ISSN: | 2095-0381 |