液相色谱-串联质谱法检测水产品中硝基呋喃类药物的残留量

目的建立液相色谱-串联质谱法（liquid chromatography-tandem mass spectrometry,LC-MS/MS）检测水产品中4种硝基呋喃类药物的残留量。方法样品中残留的硝基呋喃类药物经乙酸乙酯提取,无水硫酸钠脱水,减压浓缩后过HLB固相萃取柱净化,经Waters XTerra C18色谱柱分离,采用乙腈和水作为流动相进行梯度洗脱,以电喷雾离子源正负离子切换和多反应选择监测模式进行质谱分析,以外标法定量。结果 4种硝基呋喃类药物在1-40 ng/m L范围内线性关系良好,相关系数均大于0.999。在添加水平为1、5和20μg/kg时,呋喃唑酮、呋喃它酮、呋喃西林和呋...

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Bibliographic Details
Published in食品安全质量检测学报 Vol. 8; no. 4; pp. 1233 - 1239
Main Author 邢丽红 李兆新 孙伟红 孙晓杰 付树林 郑关超
Format Journal Article
LanguageChinese
Published 中国水产科学研究院黄海水产研究所,青岛266071 2017
农业部水产品质量安全检测与评价重点实验室,青岛266071
农业部水产品质量安全风险评估实验室(青岛),青岛 266071
Subjects
残留
水产品
液相色谱-串联质谱法
硝基呋喃类药物
残留
液相色谱-串联质谱法
residues
liquid chromatography-tandem mass spectrometry
aquatic products
硝基呋喃类药物
nitrofurans
水产品
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ISSN2095-0381

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Summary:目的建立液相色谱-串联质谱法（liquid chromatography-tandem mass spectrometry,LC-MS/MS）检测水产品中4种硝基呋喃类药物的残留量。方法样品中残留的硝基呋喃类药物经乙酸乙酯提取,无水硫酸钠脱水,减压浓缩后过HLB固相萃取柱净化,经Waters XTerra C18色谱柱分离,采用乙腈和水作为流动相进行梯度洗脱,以电喷雾离子源正负离子切换和多反应选择监测模式进行质谱分析,以外标法定量。结果 4种硝基呋喃类药物在1-40 ng/m L范围内线性关系良好,相关系数均大于0.999。在添加水平为1、5和20μg/kg时,呋喃唑酮、呋喃它酮、呋喃西林和呋喃妥因的平均回收率在88.3%-102%之间,批内和批间变异系数均低于15%。4种硝基呋喃类药物的检出限均为0.50μg/kg。结论该方法快速、准确、灵敏,可满足水产品中硝基呋喃类药物残留量的检测。
Bibliography:XING Li-Hong1,2,3, LI Zhao-Xin1,2,3, SUN Wei-Hong1,2,3, SUN Xiao-Jie1,2,3, FU Shu-Lin1,2,3, ZHENG Guan-Chao1,2,3（1Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; 2. Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Qingdao 266071, China; 3. Laboratory of Quality ＆ Safety Risk Assessment for Aquatic Products （Qingdao）, Ministry of Agriculture, Qingdao 266071, China）
11-5956/TS
nitrofurans;liquid chromatography-tandem mass spectrometry;aquatic products;residues
Objective To establish a method for simultaneous determination of four kinds of nitrofurans residues in aquatic products by liquid chromatography-tandem mass spectrometry （LC-MS/MS）.Methods The residues of nitrofurans in samples were extracted with ethyl acetate,and dehydrated with anhydrous sodium sulfate.Then the extracts were concentrated in vacuum condition,and purified on HLB solid phase extraction column.The residues of nitrofurans in the extra
ISSN:2095-0381