葛根多糖的体外抗氧化活性研究

目的考察葛根多糖的体外抗氧化作用。方法从葛根中提取多糖,苯酚-硫酸法测定多糖含量。分别研究葛根粗多糖和精制多糖对DPPH、O2^-·、·OH和NO2-的清除作用,并与阳性对照VC进行比较,考察葛根多糖的抗氧化效果。结果葛根粗多糖中多糖含量为3.67%,精制多糖中多糖含量为8.74%。在0.5~4.0mg/mL浓度范围内,葛根粗多糖和精制多糖对DPPH、O2^-·、·OH和NO2^-均有不同程度的清除作用,清除活性顺序为·OH〉O2^-·〉NO2^-〉DPPH,且清除效果与多糖浓度呈剂量-效应关系,但清除率均低于阳性对照VC。在清除自由基时,相同浓度的葛根粗多糖的清除能力强于精制多糖。结论葛根多...

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Published in食品安全质量检测学报 Vol. 8; no. 8; pp. 3050 - 3055
Main Author 李化强 吴菲菲 龙艳珍 赵良忠 徐永平 李淑英
Format Journal Article
LanguageChinese
Published 湖南省果蔬清洁加工工程技术研究中心, 邵阳 422000%大连理工大学生命科学与生物技术学院, 大连 116024 2017
邵阳学院食品与化学工程学院, 邵阳 422000
湖南省果蔬清洁加工工程技术研究中心, 邵阳 422000
大连赛姆生物工程技术有限公司博士后科研工作站, 大连 116620%邵阳学院食品与化学工程学院, 邵阳 422000
大连理工大学生命科学与生物技术学院, 大连 116024
大连赛姆生物工程技术有限公司博士后科研工作站, 大连 116620%大连赛姆生物工程技术有限公司博士后科研工作站,大连,116620
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ISSN2095-0381

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Summary:目的考察葛根多糖的体外抗氧化作用。方法从葛根中提取多糖,苯酚-硫酸法测定多糖含量。分别研究葛根粗多糖和精制多糖对DPPH、O2^-·、·OH和NO2-的清除作用,并与阳性对照VC进行比较,考察葛根多糖的抗氧化效果。结果葛根粗多糖中多糖含量为3.67%,精制多糖中多糖含量为8.74%。在0.5~4.0mg/mL浓度范围内,葛根粗多糖和精制多糖对DPPH、O2^-·、·OH和NO2^-均有不同程度的清除作用,清除活性顺序为·OH〉O2^-·〉NO2^-〉DPPH,且清除效果与多糖浓度呈剂量-效应关系,但清除率均低于阳性对照VC。在清除自由基时,相同浓度的葛根粗多糖的清除能力强于精制多糖。结论葛根多糖具有良好的抗氧化活性,可开发成新型的葛根源保健品或天然绿色的食品抗氧化剂。
Bibliography:11-5956/TS
Puerariae Radix;polysaccharides;antioxidative effect;free radicals;scavenging ability
Objective To explore antioxidative effects of polysaccharides of Puerariae Radix in vitro. Methods The polysaccharides were extracted from Puerariae Radix using ultrasonic extraction technology and detected by phenol-sulfuric acid method. Compared with the VC positive control, the scavenging activity of crude and refined polysaccharides of Puerariae Radix were studied, including DPPH, O2 ^- ·, ·OH and NO2^ - . Results The polysaccharide contents in crude and refined extracts were 3.67% and 8.74%, respectively. The results showed that the crude and refined polysaccharides of Puerariae Radix had scavenging capacity in different degrees on the DPPH, O2^-·, ·OH and NO2^-, and the order of scavenging activity was ·OH〉O2-·〉NO2-〉DPPH. In the range of 0.5~4.0 mg/mL, the scavenging effects were in a dose-effect relationship with the concentration of polysaccharides. The scavenging ability against 4 kinds of free radicals of
ISSN:2095-0381