高效液相色谱法测定黄瓜和番茄中苯丁锡残留量
目的建立黄瓜、西红柿中苯丁锡残留量的检测方法。方法样品采用二氯甲烷：乙腈（V：V=1：2）溶液振摇提取,用N-丙基乙二胺（PSA）作为净化剂净化,采用PhenomenexKinetex EVO C（18）柱（4.6 mm×150 mm,5μm）,以乙腈：0.01 mol/L盐酸（含0.6%氯化钠）（V：V=85：15）为流动相等度洗脱分离,流速1.0 mL/min,在220 nm波长处用高效液相色谱法检测。结果苯丁锡在0.5-50mg/L范围内线性关系良好。平均回收率为92.9%-98.6%,相对标准偏差（RSD）在0.7%-5.0%之间（n=6）,检出限为0.05 mg/kg。结论该方法操作...
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| Published in | 食品安全质量检测学报 Vol. 7; no. 7; pp. 2671 - 2676 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
上海市食品药品检验所,上海,201203
2016
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| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-0381 |
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| Summary: | 目的建立黄瓜、西红柿中苯丁锡残留量的检测方法。方法样品采用二氯甲烷：乙腈（V：V=1：2）溶液振摇提取,用N-丙基乙二胺（PSA）作为净化剂净化,采用PhenomenexKinetex EVO C（18）柱（4.6 mm×150 mm,5μm）,以乙腈：0.01 mol/L盐酸（含0.6%氯化钠）（V：V=85：15）为流动相等度洗脱分离,流速1.0 mL/min,在220 nm波长处用高效液相色谱法检测。结果苯丁锡在0.5-50mg/L范围内线性关系良好。平均回收率为92.9%-98.6%,相对标准偏差（RSD）在0.7%-5.0%之间（n=6）,检出限为0.05 mg/kg。结论该方法操作简便、准确、可靠,可用于黄瓜、西红柿等蔬菜中苯丁锡残留量的测定。 |
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| Bibliography: | 11-5956/TS Objective To develop a method for determination of fenbutatin oxide in cucumbers and tomatoes. Methods The vegetable samples were extracted with dispersive solid-phase extraction （d-SPE） of dichloromethane/ acetonitrile （V：V=1：2）, and cleaned up using PSA, the extracts were separated with Phenomenex Kinetex EVO C-8 column （4.6 mm×150 mm, 5μm）, using acetonitrile and 0.01 mol/L hydrochloric acid solution （0.6% sodium chloride） （V：V=85：15） as mobile phase under 1.0 mL/min flow rate. Finally, the extracts were analyzed by high performance liquid chromatography （HPLC） with 220 nm. Results It had a good linearity of fenbutatin oxide in 0.5 - 50 mg/L. The average recoveries were in range of 92.9%-98.6%, the relative standard deviations （RSD） were 0.7%-5.0%（n=6） and the limits of detection were 0.05 mg/kg, respectively. Conclusion This method is simple, accurate and reliable, which is suitable for the determination of fenbutatin oxide in cucumbers and tomatoes. LI Wen-Ting, MIAO Shui, LU Ji-Wei, CHEN Ming, |
| ISSN: | 2095-0381 |