基于DNA测序方法检测生鲜肉中5种动物源性成分

目的建立生鲜肉中猪、牛、羊、鸡、鸭源性成分的DNA测序鉴别方法,并对采集自北京各地区生鲜肉品进行检测验证。方法合成动物线粒体上12S rRNA、cytochrome b、cytochrome c oxidase基因引物,建立猪、牛、羊、鸡、鸭源性成分的DNA测序鉴别方法,并对50份猪、牛、羊、鸡、鸭肉品进行检测。结果使用线粒体上12S rRNA、cytochrome b、cytochrome c oxidase基因引物对猪、牛、羊、鸡、鸭肉品进行扩增,分别获得456、400和710 bp大小的DNA片段。只有12S rRNA基因引物均可对此5种动物源性成分扩增且效果良好。扩增产物进行序列测定。...

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Bibliographic Details
Published in食品安全质量检测学报 Vol. 8; no. 7; pp. 2394 - 2400
Main Author 任秀 仝伟建 骆海朋 谢冠东 陈怡文 刘娜 李佳铱 余文 崔生辉
Format Journal Article
LanguageChinese
Published 中国食品药品检定研究院,北京,100050%甘肃省食品检验研究院,兰州,730030%北京三药科技开发公司,北京,100176 2017
Subjects
动物源性成分
测序
系统进化树
线粒体
肉类掺假
肉类掺假
phylogenetic trees
系统进化树
animal-derived components
meat adulteration
测序
动物源性成分
线粒体
mitochondrion
sequencing
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ISSN2095-0381

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Summary:目的建立生鲜肉中猪、牛、羊、鸡、鸭源性成分的DNA测序鉴别方法,并对采集自北京各地区生鲜肉品进行检测验证。方法合成动物线粒体上12S rRNA、cytochrome b、cytochrome c oxidase基因引物,建立猪、牛、羊、鸡、鸭源性成分的DNA测序鉴别方法,并对50份猪、牛、羊、鸡、鸭肉品进行检测。结果使用线粒体上12S rRNA、cytochrome b、cytochrome c oxidase基因引物对猪、牛、羊、鸡、鸭肉品进行扩增,分别获得456、400和710 bp大小的DNA片段。只有12S rRNA基因引物均可对此5种动物源性成分扩增且效果良好。扩增产物进行序列测定。根据序列构建的系统进化树发现,此方法可有效区分样品中猪、牛、羊、鸡、鸭源性成分。结论此方法快速、简便,可准确检测50种生鲜肉样品中的动物源性成分,作为肉类鉴别的新方法。
Bibliography:11-5956/TS
mitochondrion; sequencing; meat adulteration; phylogenetic trees; animal-derived components
Objective To establish the DNA sequencing method for identification of pork, cattle, sheep, chicken and duck in raw meat, and to test and verify the fresh meat purchased from Beijing regions. Methods The primers of 12S rRNA gene, cytochrome b gene and cytochrome c oxidase gene were synthesized, DNA sequencing and identification methods of pork, cattle, sheep, chicken and duck origin were established, and 50 kinds of meat samples of pork, cattle, sheep, chicken and duck were tested. Results The primers of 12S rRNA gene, cytochrome b gene and cytochrome c oxidase gene on mitochondria were used to amplify meat samples of pork, cattle, sheep, chicken and duck, and 456、400 and 710 bp DNA fragments were obtained, respectively. Only primers on 12S rRNA genes could amplify the 5 kinds of animal-derived components, and the effect was good. Amplification products were sequenced. According to phylogenetic tree constructe
ISSN:2095-0381