CRISPR/Cas9 offers a new tool for studying the role of chromatin architecture in disease pathogenesis

• Published in: Genome Biology Vol. 19; no. 1; p. 185
• Main Authors: Guo, Xiang, Dean, Ann
• Format: Journal Article
• Language: English
• Published: London BioMed Central 06.11.2018; Springer Nature B.V; BMC
• Subjects: Animal Genetics and Genomics; Bioinformatics; Biomedical and Life Sciences; CCCTC-Binding Factor - genetics; CCCTC-Binding Factor - metabolism; Chromatin; Chromosomes; CRISPR; CRISPR-Cas Systems; Deoxyribonucleic acid; Disease; DNA; Evolutionary Biology; Gene expression; Gene Expression Regulation, Neoplastic; Genetic engineering; genome; Genomes; Human Genetics; Humans; Insights from Genome Editing; Life Sciences; Male; Microbial Genetics and Genomics; pathogenesis; Plant Genetics and Genomics; Prostate cancer; prostatic neoplasms; Prostatic Neoplasms - genetics; Prostatic Neoplasms - pathology; Protein Binding; Proteins; Regulatory Elements, Transcriptional; Regulatory sequences; Research Highlight; Long-range Looping; Topologically Associating Domains (TADs); CCCTC-binding Factor (CTCF); CTCF Sites; Insulator Protein CTCF
• ISSN: 1474-760X; 1474-7596; 1474-760X
• DOI: 10.1186/s13059-018-1569-z

A recent study used CRISPR/Cas9 to reveal long-range looping between disease-related genes and their regulatory elements that is mediated by the CCCTC-binding factor (CTCF) in prostate cancer.A recent study used CRISPR/Cas9 to reveal long-range looping between disease-related genes and their regulatory elements that is mediated by the CCCTC-binding factor (CTCF) in prostate cancer.