采用吸宫和电凝方法建立小鼠子宫内膜损伤模型的比较

[摘要]目的：比较吸宫和电凝两种方法造成小鼠子宫内膜损伤模型的特点。方法：吸宫组采用自制20G针头插入ICR小鼠一侧宫腔，以0．05MPa负压全面吸宫1周；电凝组采用自制单极电针以0．5W功率迅速电凝损伤子宫腔。均以对侧子宫为对照，对照仅插入针头或电针后退出。比较平均手术时间、术后单层子宫内膜厚度、妊娠4d子宫内膜容受性相关因子的表达情况、妊娠10d胎鼠数差别。其中HE染色观察子宫内膜组织形态学改变，蛋白质印迹法检测子宫内膜白血病抑制因子和抑瘤素M的表达。结果：吸宫组平均手术时间（10．2±1．3）min，电凝组平均手术时间（10．1±1．5）min，差异无统计学意义（P〉0．05）。吸宫组损...

Full description

Saved in:
Bibliographic Details
Published in浙江大学学报（医学版） Vol. 46; no. 2; pp. 186 - 191
Main Author 王晏鹏 黄琼晓 徐盛 舒静
Format Journal Article
LanguageChinese
Published 浙江省人民医院(杭州医学院附属人民医院)生殖内分泌科,浙江杭州310014 2017
浙江省人民医院(杭州医学院附属人民医院)妇科,浙江杭州,310014%浙江省人民医院(杭州医学院附属人民医院)生殖内分泌科,浙江杭州,310014%浙江省人民医院(杭州医学院附属人民医院)妇科,浙江杭州310014
Subjects
人工／方法
动物
子宫内膜
子宫疾病
流产
电凝术
疾病模型
黏连
Electrocoagulation
Adhesions
电凝术
Abortion,induced/methods
子宫内膜
流产,人工/方法
Uterine diseases
子宫疾病
黏连
Endometrium
疾病模型,动物
Disease models,animal
Online AccessGet full text
ISSN1008-9292
DOI10.3785/j.issn.1008-9292.2017.04.11

Cover

More Information
Summary:[摘要]目的：比较吸宫和电凝两种方法造成小鼠子宫内膜损伤模型的特点。方法：吸宫组采用自制20G针头插入ICR小鼠一侧宫腔，以0．05MPa负压全面吸宫1周；电凝组采用自制单极电针以0．5W功率迅速电凝损伤子宫腔。均以对侧子宫为对照，对照仅插入针头或电针后退出。比较平均手术时间、术后单层子宫内膜厚度、妊娠4d子宫内膜容受性相关因子的表达情况、妊娠10d胎鼠数差别。其中HE染色观察子宫内膜组织形态学改变，蛋白质印迹法检测子宫内膜白血病抑制因子和抑瘤素M的表达。结果：吸宫组平均手术时间（10．2±1．3）min，电凝组平均手术时间（10．1±1．5）min，差异无统计学意义（P〉0．05）。吸宫组损伤侧宫腔无封闭现象，电凝组有2只小鼠存在宫腔局部封闭、远段积水。损伤侧子宫内膜厚度吸宫组（96．1±13．2）μm、电凝组（88．9±16．8）μm，均比对照侧子宫减小（均P〈0．01）。损伤侧子宫内膜白血病抑制因子和抑瘤素M的表达吸宫组高于电凝组，但两组损伤侧表达均低于对照侧（均P〈0．01）。吸宫组损伤侧平均胎鼠数目（4．2±0．9）只，电凝组损伤侧平均胎鼠数目（3．9±1．7）只，均较对照侧减少（均P〈0．01）。电凝组损伤侧还可见胎鼠死亡现象。结论：吸宫和电凝均可造成小鼠子宫内膜损伤，导致子宫内膜容受性下降和生育力受损。电凝损伤更符合重度宫腔粘连特征，而吸宫损伤可能更适用于轻中度宫腔粘连研究。
Bibliography:33-1248/R
Objective： To establish mouse endometrial injury model by curettage or coagulation. Methods： Female ICR mice were randomly allocated into 2 groups： in curettage group, a blunt 20 G needle was inserted into one uterine horn with 0.05 megapascals negative pressure; in coagulation group, one uterine horn was coagulated by a monopolar electric needle with 0.5 watts power. In both groups, the contra-lateral uterine horn was used as control. The morphological changes and thickness of endometrium were evaluated 1 week after operation. The endometrial samples were taken on d 4 of pregnancy, and the expressions of endometrial receptivity-related cytokines were examined. The number of implanted embryos on each side of uterus was calculated on d 10 of pregnancy. Results： There was no difference in operation time between 2 groups. In both groups, the endometrial glands and stroma were significantly reduced, and the endometrial thickness also significantly decreased on injury side compared to contra-lateral horn.
ISSN:1008-9292
DOI:10.3785/j.issn.1008-9292.2017.04.11