过氧化物酶—分光光度法测定小麦粉中的过氧化钙

目的建立过氧化物酶-分光光度法测定小麦粉中过氧化钙含量的方法。方法稀硫酸与样品混合反应,然后用碱液中和,离心分离后得到的上清液,与过氧化物酶溶液、4-氨基安替比林与苯酚的混合磷酸缓冲液水浴显色,于505 nm处测定吸光度。结果本方法回收率96.6%-99.0%,方法检出限和定量限分别为7.4和22.0 mg/kg,线性范围0.4-10μg/m L,r-2为0.9980。结论本方法简单、可靠、准确,适用于小麦粉中过氧化钙含量的测定。...

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Bibliographic Details
Published in食品安全质量检测学报 Vol. 7; no. 8; pp. 3376 - 3381
Main Author 宋桂雪 刘宁 张溪 杨柏崇 宋薇
Format Journal Article
LanguageChinese
Published 谱尼测试集团股份有限公司,北京,100080 2016
Subjects
分光光度法
禁用添加剂
过氧化物酶
过氧化钙
面粉
面粉
过氧化物酶
prohibited additive
禁用添加剂
calcium peroxide,peroxidase,spectrophotometry
分光光度法
wheat flour
过氧化钙
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ISSN2095-0381

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Summary:目的建立过氧化物酶-分光光度法测定小麦粉中过氧化钙含量的方法。方法稀硫酸与样品混合反应,然后用碱液中和,离心分离后得到的上清液,与过氧化物酶溶液、4-氨基安替比林与苯酚的混合磷酸缓冲液水浴显色,于505 nm处测定吸光度。结果本方法回收率96.6%-99.0%,方法检出限和定量限分别为7.4和22.0 mg/kg,线性范围0.4-10μg/m L,r-2为0.9980。结论本方法简单、可靠、准确,适用于小麦粉中过氧化钙含量的测定。
Bibliography:wheat flour; calcium peroxide; peroxidase; spectrophotometry; prohibited additive
11-5956/TS
Objective To establish a method for determination of calcium peroxide in wheat flour by peroxidase-spectrophotometry. Methods The sample was mixed with diluted sulfuric acid, neutralized by alkaline liquid, and the supernatant was achieved after centrifugal separation. Then, the supernatant was mixed with the solution of peroxidase, phosphate buffer containing 4-amino antipyrine and phenol, and the mixture was transferred to water bath to make colorimetric reaction. Finally, the absorbance was measured at 505 nm by colorimetry. Results The spiked recoveries were in the range of 96.6%-99.0%, the limit of detection and limit of quantification were 7.4 and 22.0 mg/kg, respectively, and the linear correlation was in the range of 0.4-10 μg/m L with r2 0.9980. Conclusion This proposed method is simple, rapid and reliable, and it is suitable for determination of calcium peroxide in wheat flour.
SONG Gui-Xue, LIU Ning, ZHANG Xi,
ISSN:2095-0381