Adhesive Strength of Autologous Fibrin Glue

• Published in: Biological & pharmaceutical bulletin Vol. 23; no. 3; pp. 313 - 317
• Main Authors: HIROZANE, Kiyoshi, YOSHIDA, Hisahiro, KAMIYA, Akira
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 01.03.2000; Maruzen; Japan Science and Technology Agency
• Subjects: ablesive strength; autologous plasma; Biological and medical sciences; Calcium Chloride - analysis; cryoprecipitate; Fibrin; fibrin glue; fibrinogen; fibronectin; General pharmacology; Humans; Materials Testing; Medical sciences; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Reproducibility of Results; Temperature; Thrombin - analysis; Tissue Adhesives - chemistry; Temperature; Pharmaceutical technology; Mechanical properties; Environmental factor; Autologous system; Glue cement; Fibronectin; Blood plasma; Fibrin; Adhesive strength; Concentration effect; Fibrinogen; Dosage form; Calcium Chlorine; Cryoprecipitate
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.23.313

To establish an easy and rapid method for measuring the adhesive strength of fibrin glue and to clarify the factor(s) most affecting the strength, a study was made on the effect of the concentration of plasma components on the strength of cryoprecipitate (Cryo) prepared from a subject's own autologous plasma to be used as fibrin glue. The adhesive strength of the Cryo was measured with various supporting materials instead of animal skin using a tester of tension and compression. The results were as follows : (1) the strength of Cryo applied to ground flat glass (4cm2) was significantly greater than that applied to clear glass, clear plastic, or smooth and flat wood chips; (2) the adhesive strength of Cryo depended on the concentration of thrombin with the optimal concentration being 50 units/ml; (3) the concentration of CaCl2 did not affect the adhesive strength of Cryo; (4) the adhesive reaction was dependent on the temperature and the adhesive strength more quickly reached a steady state at 37°C than at lower temperature; (5) the adhesive strength was correlated well with the total concentration of fibrinogen and fibronectin. These results indicate that the adhesive strengh of Cryo can be easily and quicky evaluated using a tester and ground glass with thrombin at 50 units/ml, and that the adhesive strength of Cryo can be predicted from the total concentration of fibrinogen and fibronoctin.