Antibodies to Borna disease virus in infected adult rats: An early appearance of anti-p10 antibody and recognition of novel virus-specific proteins in infected animal brain cells

• Published in: Journal of Veterinary Medical Science Vol. 62; no. 7; pp. 775 - 778
• Main Authors: Watanabe, M. (Osaka Univ., Suita (Japan). Research Inst. for Microbial Diseases), Kobayashi, T, Tomonaga, K, Ikuta, K
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.07.2000
• Subjects: animal brain; Animals; ANTIBODIES; Antibodies, Monoclonal; Antibodies, Viral - blood; antibody; Antigens, Viral - analysis; Antigens, Viral - immunology; Blotting, Western; Borna Disease - blood; Borna Disease - immunology; BORNA DISEASE VIRUS; Borna disease virus - immunology; Borna disease virus - isolation & purification; BRAIN; Brain - virology; CELLS; Cells, Cultured; INFECTION; PROTEINS; RATS; Rats, Inbred Lew; Time Factors
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.62.775

The time course for appearance of antibodies to Borna disease virus (BDV) major antigens, p40, p24, p18 and p10 were investigated in BDV-inoculated adult rats by Western blotting. Anti-p10 antibodies were detected in sera as early as anti-p40 and -p24 antibodies at four or five weeks after inoculation. Furthermore, in addition to these major antigens of BDV, the rat serum could detect additional 80-, 58-, 43-, 20-, and 16-kDa proteins in BDV-infected cultured cells and/or animal brain cells by Western blot analysis. Of these proteins, the 20- and 16-kDa proteins were shown to be related to p24 protein by their reactivity with anti-p24 monoclonal antibody. Interestingly, the 58- and 24-kDa were found only in BDV-infected animal brain cells but not in cultured cells. The results in this study could provide a useful information on the mechanism for the viral replication and pathogenesis.