Japanese encephalitis virus infection in non-encephalitic acute febrile illness patients

• Published in: PLoS neglected tropical diseases Vol. 14; no. 7; p. e0008454
• Main Authors: Ma’roef, Chairin Nisa, Dhenni, Rama, Megawati, Dewi, Fadhilah, Araniy, Lucanus, Anton, Artika, I Made, Masyeni, Sri, Lestarini, Asri, Sari, Kartika, Suryana, Ketut, Yudhaputri, Frilasita A., Jaya, Ungke Anton, Sasmono, R. Tedjo, Ledermann, Jeremy P., Powers, Ann M., Myint, Khin Saw Aye
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 14.07.2020; Public Library of Science (PLoS)
• Subjects: Algorithms; Antibodies; Antibodies, Viral - blood; Antigens; Biology and Life Sciences; Cohorts; Dengue; Dengue fever; Diagnosis; Diagnostic systems; Disease control; Disease prevention; Distribution; Emergency medical care; Encephalitis; Encephalitis Virus, Japanese; Encephalitis, Japanese - diagnosis; Encephalitis, Japanese - epidemiology; Encephalitis, Japanese - virology; Ethics; Fever; Fever - diagnosis; Fever - epidemiology; Fever - virology; Health sciences; Hematocrit; Hospitals; Human diseases; Humans; Illnesses; Immunoglobulin M; Immunoglobulin M - blood; Indonesia - epidemiology; Infections; Invasiveness; Japanese encephalitis; Laboratories; Leukopenia; Medicine; Medicine and Health Sciences; Molecular biology; Neutralization; Patients; People and Places; Research and Analysis Methods; Serologic Tests; Serology; Serum; Supervision; Thrombocytopenia; Tropical diseases; Vector-borne diseases; Vectors (Biology); Viruses; Indonesia; Jakarta Indonesia; Thailand; United States > US; Colorado
• ISSN: 1935-2735; 1935-2727; 1935-2735
• DOI: 10.1371/journal.pntd.0008454

Although Japanese encephalitis virus (JEV) is considered endemic in Indonesia, there are only limited reports of JEV infection from a small number of geographic areas within the country with the majority of these being neuroinvasive disease cases. Here, we report cases of JEV infection in non-encephalitic acute febrile illness patients from Bali, Indonesia. Paired admission (S1) and discharge (S2) serum specimens from 144 acute febrile illness patients (without evidence of acute dengue virus infection) were retrospectively tested for anti-JEV IgM antibody and confirmed by plaque reduction neutralization test (PRNT) for JEV infection. Twenty-six (18.1%) patients were anti-JEV IgM-positive or equivocal in their S2 specimens, of which 5 (3.5%) and 8 (5.6%) patients met the criteria for confirmed and probable JEV infection, respectively, based on PRNT results. Notably, these non-encephalitic JE cases were less likely to have thrombocytopenia, leukopenia, and lower hematocrit compared with confirmed dengue cases of the same cohort. These findings highlight the need to consider JEV in the diagnostic algorithm for acute febrile illnesses in endemic areas and suggest that JEV as a cause of non-encephalitic disease has likely been underestimated in Indonesia.