鞘内注射经外源性BDNF活化的星形胶质细胞对正常大鼠痛觉的影响
目的探讨外源性脑源性神经营养因子(BDNF)对星形胶质细胞的活化作用,分析活化的星形胶质细胞鞘内注射与正常大鼠痛觉过敏的关系。方法体外培养的原代星形胶质细胞以外源性BDNF(100 ng/mL)分别孵育0(基线)、15、60、120 min,Western blotting检测细胞内纤丝酸性蛋白(GFAP)的表达。24只雄性SD大鼠经鞘内置管后随机分为活化细胞注射组(鞘内注射经BDNF孵育15 min的星形胶质细胞)、阴性对照组(鞘内注射未经BDNF孵育的星形胶质细胞)、安慰剂注射组(鞘内注射PBS)和空白对照组(未行鞘内注射),每组6只;分别于单次注射前和注射后0.5、2、4和8 h时间点,...
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Published in | 上海交通大学学报(医学版) Vol. 32; no. 7; pp. 881 - 885 |
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Main Author | |
Format | Journal Article |
Language | Chinese |
Published |
上海交通大学附属第六人民医院疼痛科,上海200233
2012
上海交通大学附属第六人民医院麻醉科,上海200233%上海交通大学附属第六人民医院疼痛科,上海,200233%上海交通大学附属第六人民医院麻醉科,上海,200233 |
Subjects | |
Online Access | Get full text |
ISSN | 1674-8115 |
DOI | 10.3969/j.issn.1674-8115.2012.07.013 |
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Summary: | 目的探讨外源性脑源性神经营养因子(BDNF)对星形胶质细胞的活化作用,分析活化的星形胶质细胞鞘内注射与正常大鼠痛觉过敏的关系。方法体外培养的原代星形胶质细胞以外源性BDNF(100 ng/mL)分别孵育0(基线)、15、60、120 min,Western blotting检测细胞内纤丝酸性蛋白(GFAP)的表达。24只雄性SD大鼠经鞘内置管后随机分为活化细胞注射组(鞘内注射经BDNF孵育15 min的星形胶质细胞)、阴性对照组(鞘内注射未经BDNF孵育的星形胶质细胞)、安慰剂注射组(鞘内注射PBS)和空白对照组(未行鞘内注射),每组6只;分别于单次注射前和注射后0.5、2、4和8 h时间点,von Frey纤维丝法测定各组大鼠50%机械痛缩足阈值(50%PWT)。结果经BDNF孵育15、60和120 min的星形胶质细胞内GFAP蛋白表达均显著高于基线值(P〈0.01)。活化细胞注射组大鼠注射后各时间点的50%PWT均较注射前显著降低(P〈0.01);阴性对照组大鼠50%PWT仅在注射后30 min时间点呈现一过性下降,与注射前比较差异有统计学意义(P〈0.01);安慰剂注射组和空白对照组大鼠注射前和注射后各时间点50%PWT比较,差异均无统计学意义(P〉0.05)。结论外源性BDNF可活化体外培养的星形胶质细胞,鞘内注射经外源性BDNF活化的星形胶质细胞可直接诱发正常大鼠的痛觉过敏。 |
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Bibliography: | ZENG Lu-lu, WANG Jmg , ZHANG Xin , ZHOU Quan-hong, JIANGWe , DU Dong-ping (1. Pain Management Center, 2. Department of Anesthesiology, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China) Objective To investigate the effects of exogenous brain-derived neurotrophic factor (BDNF) on the activation of astrocytes, and analyse the impact of intrathecal injection of activated astrocytes on pain hyperalgia in normal rats. Methods Primary astroeytes cultured in vitro were incubated with exogenous BDNF ( 100 ng/mL) for 0 rain (baseline), 15 min, 60 min and 120 min, and the expression of glial filament acidic protein (GFAP) in ceils was detected by Western blotting. Twenty-four male SD rats were randomly divided into activated-astrocytes group (intrathecal injection of astrocytes incubated with BDNF for 15 rain), negative control group (intrathecal injection of astrocytes without incubation with BDNF), placebo group ( intrathecal injection of PBS) and blank control group after intrathecal c |
ISSN: | 1674-8115 |
DOI: | 10.3969/j.issn.1674-8115.2012.07.013 |